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N0252

Sigma-Aldrich

4-Nitrophenyl decanoate

lipase substrate, ≥98% (TLC), powder

Synonym(s):

4-Nitrophenyl caprate, Decanoic acid 4-nitrophenyl ester

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About This Item

Empirical Formula (Hill Notation):
C16H23NO4
CAS Number:
Molecular Weight:
293.36
Beilstein/REAXYS Number:
1915230
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83

product name

4-Nitrophenyl decanoate, lipase substrate

assay

≥98% (TLC)

form

powder

solubility

chloroform: soluble 100 mg/mL, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CCCCCCCCCC(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C16H23NO4/c1-2-3-4-5-6-7-8-9-16(18)21-15-12-10-14(11-13-15)17(19)20/h10-13H,2-9H2,1H3

InChI key

RRNKCSIEGPOIKI-UHFFFAOYSA-N

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Application

4-Nitrophenyl decanoate has been used as a substrate for assessing the lipase activity spectrophotometrically. It has also been used as a substrate for estimating the carboxylesterases activity.
4-Nitrophenyl decanoate has been used as a substrate to assess the carboxylesterase production by recombinant Penicillium griseoroseum T55 strain. It has also been used for general esterase activity assay of the hepatopancreas extract.

Biochem/physiol Actions

4-Nitrophenyl decanoate is a substrate for lipase enzyme. The extracellular lipase has greater hydrolysis reaction velocity with 4-nitrophenyl decanoate when compared with other 4-nitropheny esters.

pictograms

Exclamation mark

signalword

Warning

hcodes

pcodes

Hazard Classifications

Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Isolation and characterization of an enzyme from the Greenshell? mussel Perna canaliculus that hydrolyses pectenotoxins and esters of okadaic acid
MacKenzie LA, et al.
Toxicon, 60(3), 406-419 (2012)
Carboxyl ester hydrolase from Penicillium expansum: cloning, characterization and overproduction by Penicillium griseoroseum
Correa TLR, et al.
Journal of Applied Microbiology, 115(1), 114-124 (2013)
V M Gumerov et al.
Prikladnaia biokhimiia i mikrobiologiia, 48(4), 376-382 (2012-10-06)
As a result of sequencing the genome of the termophilic alkali-tolerant lipolytic bacterium Thermosyntropha lipolytica, the gene encoding a lipase secreted into the medium was identified. The recombinant enzyme was expressed in Escherichia coli. It was isolated, purified, and functionally
Methods in Molecular biology: Platelets and Megakaryocytes, volume 1, functional assays, 27-27 (2012)
Kamila Dmochowska et al.
Journal of insect science (Online), 13, 77-77 (2013-11-14)
Bees of the genus Osmia are being used in crop pollination at an increasing rate. However, a short life expectancy of adult individuals limits the feasibility of their use. Cocoons of the red mason bee, Osmia rufa L. (Hymenoptera: Megachilidae)

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