C0794
Chitosanase from Streptomyces sp.
buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)
Synonym(s):
Chitosan N-acetylglucosaminohydrolase
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10 UNITS
$475.00
$475.00
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10 UNITS
$475.00
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
$475.00
In StockDetails
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form
buffered aqueous glycerol solution
Quality Level
specific activity
≥15 units/mg protein (E1%)
concentration
≥0.5 mg/mL
solubility
0.1 M acetate buffer, pH 5.5: soluble
storage temp.
−20°C
Application
Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.[1]
Biochem/physiol Actions
Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.
Unit Definition
One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.
Physical form
Solution in 50% glycerol containing 50 mM sodium acetate
Preparation Note
Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5[1]
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Chitosanase was produced by the strain of Streptomyces lividans TK24 bearing the csn gene from Streptomyces sp. N174, and purified by S-Sepharose and Bio-Gel A column chromatography. Partially (25-35%) N-acetylated chitosan was digested by the purified chitosanase, and structures of
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