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Merck
  • Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium.

Long-term, hormone-responsive organoid cultures of human endometrium in a chemically defined medium.

Nature cell biology (2017-04-11)
Margherita Y Turco, Lucy Gardner, Jasmine Hughes, Tereza Cindrova-Davies, Maria J Gomez, Lydia Farrell, Michael Hollinshead, Steven G E Marsh, Jan J Brosens, Hilary O Critchley, Benjamin D Simons, Myriam Hemberger, Bon-Kyoung Koo, Ashley Moffett, Graham J Burton
摘要

In humans, the endometrium, the uterine mucosal lining, undergoes dynamic changes throughout the menstrual cycle and pregnancy. Despite the importance of the endometrium as the site of implantation and nutritional support for the conceptus, there are no long-term culture systems that recapitulate endometrial function in vitro. We adapted conditions used to establish human adult stem-cell-derived organoid cultures to generate three-dimensional cultures of normal and decidualized human endometrium. These organoids expand long-term, are genetically stable and differentiate following treatment with reproductive hormones. Single cells from both endometrium and decidua can generate a fully functional organoid. Transcript analysis confirmed great similarity between organoids and the primary tissue of origin. On exposure to pregnancy signals, endometrial organoids develop characteristics of early pregnancy. We also derived organoids from malignant endometrium, and so provide a foundation to study common diseases, such as endometriosis and endometrial cancer, as well as the physiology of early gestation.

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青链霉素, with 10,000 units penicillin and 10 mg streptomycin per mL in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture
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分散酶®II, protease
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蛋白酶 K 来源于林伯氏白色念球菌, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL
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IgG 来源于人类血清, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
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胶原酶 来源于溶组织梭菌, Type V, ≥1 FALGPA units/mg solid, >125 CDU/mg solid
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核糖核酸酶A 来源于牛胰腺, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
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β-Estradiol-Water Soluble, BioReagent, suitable for cell culture
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Progesterone-Water Soluble, powder, BioReagent, suitable for cell culture
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福尔马林,10%中性缓冲液,冬青气味, histological fixative, pH 6.9-7.1
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SIGMAFAST 3,3′-二氨基联苯胺片, tablet, To prepare 1 mL
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p-Xylene-bis(N-pyridinium bromide), ≥95% (TLC)