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Merck

Culturing and Cryobanking Human Neural Stem Cells.

Methods in molecular biology (Clifton, N.J.) (2017-03-30)
Jeremy M Crook, Eva Tomaskovic-Crook
摘要

The discovery and study of human neural stem cells has advanced our understanding of human neurogenesis, and the development of novel therapeutics based on neural cell replacement. Here, we describe methods to culture and cryopreserve human neural stem cells (hNSCs) for expansion and banking. Importantly, the protocols ensure that the multipotency of hNSCs is preserved to enable differentiation to neurons and supporting neuroglia.

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Sigma-Aldrich
白蛋白 来源于人类血清, lyophilized powder, ≥97% (agarose gel electrophoresis)
Sigma-Aldrich
ReNcell CX人神经祖细胞系, ReNcell CX is an immortalized human neural progenitor cell line with the ability to readily differentiate into neurons & glial cells.