技術
HPLC: suitable
測試參數
column: Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777164)
mobile phase: [A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5
gradient: top (0.8 mL/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 mL/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min
flow rate: 0.8 mL/min or 1.5 mL/min
detector: UV 280 nm
injection: 20 μL
sample: MAb 321, 5 mg/mL
適合性
application for HPLC
應用
life science and biopharma
分析報告
Due to the low pressure of Proteomix SCX, faster analysis and high resolution can be achieved with a higher flow rate.
法律資訊
Proteomix is a registered trademark of Sepax Technologies
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