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G005559

HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: SUPELCOSIL LC-PAH, 15 cm x 4.6 mm I.D., 5 μm particles (58318)
mobile phase: [A] water, [B] methanol
gradient: 55 to 100% B in 5 min; held at 100%B for 10 min
flow rate: 1.5 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 5 μL
sample: EPA 610 mix

適合性

application for HPLC

應用

environmental

分析報告

PAHs are traditionally evaluated using an acetonitrile/water gradient. Recently customers are looking for alternatives to this method using a methanol/water gradient due to the risk of an another acetonitrile shortage, solvent costs, and toxicity to the environment. This analysis demostrates the separation using a methanol gradient.

法律資訊

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

分析物

描述
    Naphthalene
    Acenaphthene
    Fluorene
    Phenanthrene
    Anthracene
    Fluoranthene
    Pyrene
    Benz[a]anthracene solution
    Chrysene
    Benzo[b]fluoranthene
    Benzo[k]fluoranthene
    Benzo[a]pyrene
    Benzo[ghi]perylene
    Indeno[1,2,3-cd]pyrene
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