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Merck
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G004414

LC/MS Analysis of Peptides on Discovery® BIO Wide Pore C18, Effect of TFA Concentration on MS Signal

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Discovery® BIO Wide Pore C18, 15 cm x 2.1 mm I.D., 3 μm particles (567202-U)
mobile phase: [A]: aqueous component (25 mM formic acid, 0.01% TFA or 0.1% TFA); [B] (50:50) water:acetonitrile containing same ionic additives as aq. component
flow rate: 0.20 mL/min
column temp.: ambient
detector: (+)ESI
injection: 1-3 μL
sample: RP Peptide Performance Standard (Alberta Peptide Inst., Cat. No. RPS-P0010)

適合性

application for HPLC

應用

life science and biopharma

法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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