Upon receiving the column, it should be flushed with 30% acetonitrile/70% water for 15 minutes at a flow rate of 1 mL/min. Subsequently, the columns should be equilibrated with the starting buffer, followed by running a blank gradient.
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產品名稱
TSKgel® DNA-NPR HPLC Column, phase DEAE (diethylaminoethyl), L × I.D. 7.5 cm × 4.6 mm, 2.5 μm particle size
材料
stainless steel hardware
產品線
TSKgel®
特點
counter ion ClO4-
製造商／商標名
Tosoh 18249
參數
≤1 M salt concentration
≤20% organic solvent
1.5 mL/min flow rate
10-60 °C temperature
300 bar max. pressure
技術
HPLC: suitable
長度 × 內徑
7.5 cm × 4.6 mm
基質
polymer particle platform
基質活性組
DEAE (diethylaminoethyl) phase
粒徑
2.5 μm
工作pH值
2-12
分離技術
anion exchange
一般說明
TSKgel DNA-NPR columns are packed with 2.5 micron, non-porous, high efficiency, hydrophilic polymer beads which are surface modified with a weak anion exchanger. Because NPR columns contain non-porous particles, binding capacity is low compared to porous columns with the same ligand functionality. Column dimensions are optimized for the high efficiency separation of DNA fragments, PCR products, or plasmids. A small guard column is available to protect the analytical column.
法律資訊
TSKgel is a registered trademark of Tosoh Corporation
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