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581373-U

Supelco

Ascentis® C18 (5 µm) HPLC Columns

L × I.D. 2 cm × 4 mm, HPLC Guard Cartridge Kit with 1 Cartridge holder + 1 Guard Cartridge

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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產品名稱

Ascentis® C18 Supelguard 保护柱芯, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm

材料

stainless steel column

品質等級

100

agency

suitable for USP L1

產品線

Ascentis®

特點

endcapped

包裝

pkg of 1 kit

標籤範圍

25% Carbon loading

參數

≤70 °C temp. range

技術

HPLC: suitable
LC/MS: suitable

長度 × 內徑

2 cm × 4 mm

表面積

450 m2/g

雜質

5 ppm metals

基質

fully porous particle

基質活性組

C18 (octadecyl) phase

粒徑

5 μm

孔徑

100 Å

工作pH值範圍

2-8

應用

food and beverages

分離技術

reversed phase

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一般說明

套件包括一个保护柱芯、一个独立式柱套、一段管路、2 个接头螺母和 2 个密封垫圈。

腳註

Kit includes one cartridge, a stand-alone holder, a piece of tubing, 2 nuts and ferrules (10mm i.d guard requires the purchase of 567499-U)

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法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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分析證明 (COA)

Lot/Batch Number

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Massimo Tacchini et al.
Journal of pharmaceutical and biomedical analysis, 146, 1-6 (2017-08-26)
The genus Echinacea (Asteraceae) includes species traditionally used in phytotherapy. Among them, Echinacea pallida (Nutt.) Nutt. root extracts are characterized by a representative antiproliferative activity, due to the presence of acetylenic compounds. In this study, supercritical fluid extraction (SFE) was

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Analysis of Polar Compounds with Ion Pair Reagents

Reversed-phase HPLC sometimes requires ion pairing for adequate retention of polar compounds due to hydrophobic interactions.

Questions

  1. Could you provide me with details on how to connect the product 581373-U to the respective column 581325-U?

    1 answer

    1. The installation process involves the following steps:
      1.Install the guard cartridge into the holder as shown in the attached document.
      2.Connect the tubing from the HPLC injector to the inlet of the guard column holder with the cone-shaped ferrule pointed down towards the end of the tubing.
      3.Install the tubing/nut/ferrule provided in the kit to the outlet of the guard column holder, following the same process as described in step no. 2.
      4.Connect the outlet tubing from the guard holder to the analytical HPLC column, again, following the same procedure as directed in step no. 2.

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