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53324-U

Supelco

Ascentis® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 4.6 mm, HPLC Column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52
暫時無法取得訂價和供貨情況

產品名稱

Ascentis® Express Peptide ES-C18, 2.7μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 10 cm × 4.6 mm

材料

stainless steel column

品質等級

100

agency

suitable for USP L1

產品線

Ascentis®

特點

endcapped: no

製造商／商標名

Ascentis®

包裝

1 ea of

參數

≤100 °C temp. range
600 bar max. pressure (9000 psi)

技術

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

長度 × 內徑

10 cm × 4.6 mm

表面積

90 m2/g

雜質

<5 ppm metals

基質

Fused-Core particle platform
superficially porous particle

基質活性組

C18 (octadecyl) phase

粒徑

2.7 μm

孔徑

160 Å

工作pH值範圍

1-9

分離技術

reversed phase

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相關類別

一般說明

Ascentis Express Peptide ES-C18 色谱柱是专为分离肽和小分子蛋白等高分子量化合物而设计的。这些色谱柱含有先进的熔融核心颗粒，这些颗粒具有较大的孔隙（标准 Ascentis Express 中的 160Å与 90Å），与立体保护的 C18 配体结合，以提供极低 pH 值 (<1) 和高温（高达 100ºC）下的额外稳定性 (ES)。这大大扩展了 Ascentis Express 色谱柱的应用范围。

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
USZH001511
USZH001510
USZH001509
USZH001508
USZH001507

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如果您需要一個特定的版本，您可以透過批號來尋找特定憑證。

搜尋 COA

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6

文章

Ascentis® Express Peptide ES-C18 U/HPLC Columns

Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.

Questions

1–10 of 12 Questions  

  1. Can product 53324-U Ascentis® Express Peptide ES C18 HPLC column be used for LC-MS?

    1 answer

    1. Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53324-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Helpful?

  2. Do I need special fittings and tubing to connect product 53324-U Ascentis® Express Peptide ES C18 HPLC ColumnExpress columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Helpful?

  3. Can I use product 53324-U Ascentis® Express Peptide ES C18 HPLC Column on any type of HPLC system?

    1 answer

    1. Product 53324-U Ascentis® Express Peptide ES C18 HPLC Column HPLC columns is capable of use on standard HPLC systems as well as UHPLC systems.  This column is packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Helpful?

  4. How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  5. Can I use product 53324-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?

    1 answer

    1. Yes.  product 53324-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, product 53324-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53324-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  6. What is special about product 53324-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 answer

    1. Product 53324-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. Is there anything special I need to do to my HPLC system to use product 53324-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 answer

    1. Nothing special is required to use product 53324-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).

      Helpful?

  9. Are guard columns available for product 53324-U Ascentis® Express Peptide ES C18 HPLC Column?

    1 answer

    1. Yes, we have guard columns available for product 53324-U Ascentis® Express Peptide ES C18 HPLC Column.  Product 53500-U is the Ascentis® Express Guard Holder and Product 53542-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 3.0 mm .

      Helpful?

  10. What flow rate should I use with product 53324-U Ascentis® Express Peptide ES C18 HPLC columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53324-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

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1–10 of 12 Questions  

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