While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
推薦產品
產品名稱
Ascentis® Express Peptide ES-C18, 2.7μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 15 cm × 3 mm
材料
stainless steel column
agency
suitable for USP L1
產品線
Ascentis®
特點
endcapped: no
製造商／商標名
Ascentis®
包裝
1 ea of
參數
≤100 °C temp. range
600 bar max. pressure (9000 psi)
技術
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
長度 × 內徑
15 cm × 3 mm
表面積
90 m2/g
雜質
<5 ppm metals
基質
Fused-Core particle platform
superficially porous particle
基質活性組
C18 (octadecyl) phase
粒徑
2.7 μm
孔徑
160 Å
工作pH值範圍
1-9
分離技術
reversed phase
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一般說明
Ascentis Express Peptide ES-C18 色谱柱是专为分离肽和小分子蛋白等高分子量化合物而设计的。这些色谱柱含有先进的熔融核心颗粒，这些颗粒具有较大的孔隙（标准 Ascentis Express 中的 160Å与 90Å），与立体保护的 C18 配体结合，以提供极低 pH 值 (<1) 和高温（高达 100ºC）下的额外稳定性 (ES)。这大大扩展了 Ascentis Express 色谱柱的应用范围。
法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
相關產品
防護柱套
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
文章
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.
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Do I need special fittings and tubing to connect product 53314-U Ascentis® Express Peptide ES C18 HPLC ColumnExpress columns?
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Is there anything special I need to do to my HPLC system to use product 53314-U Ascentis® Express Peptide ES C18 HPLC Column?
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Nothing special is required to use product 53314-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
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What is the carbon load for product 53314-U Ascentis® Express Peptide ES C18 HPLC Column?
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Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for product 53314-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.
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Can I use product 53314-U Ascentis® Express Peptide ES C18 HPLC Column on any type of HPLC system?
1 answer-
Product 53314-U Ascentis® Express Peptide ES C18 HPLC Column HPLC columns is capable of use on standard HPLC systems as well as UHPLC systems. This column is packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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What is special about product 53314-U Ascentis® Express Peptide ES C18 HPLC Column?
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Product 53314-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What flow rate should I use with product 53314-U Ascentis® Express Peptide ES C18 HPLC columns?
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Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53314-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
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The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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What is the pressure rating for product 53314-U Ascentis® Express Peptide ES C18 HPLC Column?
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This product 53314-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)
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Can I use product 53314-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?
1 answer-
Yes. product 53314-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, product 53314-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53314-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Helpful?
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