ZRB1625

Anti-FAK Antibody, clone 1D14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: EC:2.7.10.2, FADK 1, FRNK, Focal adhesion kinase 1, Focal adhesion kinase-related nonkinase, PPP1R71, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1D14, monoclonal; recombinant monoclonal
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 119.23 kDa; observed mol wt ~120 kDa
• 物種活性: human, rat
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• UniProt登錄號: Q05397
• 環保替代類別: Aligned,
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTK2(5747)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特異性

Clone 1D14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Focal adhesion kinase 1. It targets an epitope within the N-terminal half.

免疫原

His-tagged recombinant fragment corresponding to the first 423 amino acids from the N-terminal region of human Focal Adhesion Kinase 1.

應用

Anti-FAK, clone 1D14 ZooMAb, Cat. No. ZRB1625, is a recombinant Rabbit monoclonal antibody that specifically detects FAK and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected human recombinant FAK .

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected FAK in human placenta and human tonsil tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected FAK in HUVEC .

Affinity Binding Assay: A representative lot of this antibody bound human recombinant FAK with a KD of 8.5 x 10-7 in an affinity binding assay. .

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Focal adhesion kinase 1 (UniProt: Q05397; also known as EC:2.7.10.2, FADK 1, Focal adhesion kinase-related nonkinase, FRNK, Protein phosphatase 1 regulatory subunit 71, PPP1R71, Protein-tyrosine kinase 2, p125FAK, pp125FAK) is encoded by the PTK2 (also known as FAK, FAK1) gene (Gene ID: 5747) in human. FAK is a non-receptor tyrosine kinase that localizes to focal adhesions and contributes to the processes of integrin-mediated cell spreading and migration. It has two nucleotide binding regions (aa 428-434 and 500-502). Its protein kinase domain is localized to amino acids 422-689. FAK 1 plays a role in in regulating cell proliferation, migration, adhesion, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, and apoptosis. It is also required for early embryonic development and placenta development. FAK is involved in integrin signal transduction and in signaling downstream of numerous growth factor receptors and G-protein coupled receptors. FAK undergoes tyrosine phosphorylated in response to various environmental stimuli. Tyrosine 397 is a major autophosphorylation site for FAK, however, it can also be phosphorylated by other kinases. Phosphorylation at tyrosine 397 promotes interaction with SRC and SRC family members, leading to phosphorylation at tyrosine 576 and 577 and at additional tyrosine residues. Phosphorylation at tyrosine 397 is also shown to be important for interaction with BMX, PIK3R1 and SHC1. Microtubule-induced dephosphorylation at tyrosine 397 is crucial for the induction of focal adhesion disassembly and is catalyzed by protein tyrosine phosphatase non-receptor type 11 (PTPN11). Aberrant expression of FAK1 is reported in cancer cell proliferation, migration and invasion, and metastasis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1