ZRB1596

Anti-Atg5 Antibody, clone 1L18 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: Autophagy protein 5
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 1L18, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 32.45 kDa; observed mol wt ~55 kDa
• 純化經由: using Protein A
• 物種活性: mouse, rat, human
• 物種活性（以同源性預測）: feline, bovine, canine, horse, monkey
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 同型: IgG
• 表位序列: C-terminal
• Protein ID登錄號: NP_001273035
• UniProt登錄號: Q9H1Y0
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ATG5(9474)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 1L18 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects ATG5. It targets an epitope within 19 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Autophagy Protein 5 (Atg5).

應用

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Atg5 in A549 cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected Atg5 in Mouse Pancreas tissue and PC12 cell lysate and 1:1000 dilution detected Atg5 in HepG2 cell lysates.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Atg5 in U251 cells treated with chloroquine.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Atg5 in human kidney tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Atg5 with a KD of 7.4 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

標靶描述

Autophagy protein 5 (UniProt: Q9H1Y0; also known as APG5-like, Apoptosis-specific protein) is encoded by the ATG5 (also known as APG5L, ASP) gene (Gene ID: 9474) in human. Autophagy is a highly regulated process that is involved in cell growth, development, and cell death. In this process cytoplasmic components are delivered to the lysosome in a very systematic manner for degradation. Atg5 is a ubiquitous autophagy factor that binds to other proteins to form multiprotein complex. In Western blotting protocols, it is detected at about 55-56 kDa range due to its association with Atg12. It is shown to be indispensable in both canonical and non-canonical autophagy. It is an integral part of the Atg5-Atg12-Atg16L1 complex that catalyzes the Atg8 lipidation essential for autophagosome formation and expansion. This complex serves as a ubiquitin-like conjugation system that contributes to the elongation of the isolated membrane and autophagosome maturation. The conjugation of Atg5 with Atg12 is catalyzed by Atg7 and Atg10. Here first Atg7 activates Atg12 and then Atg12 is transferred to Atg10 (an E2-like enzyme), and finally it conjugates to Atg5. This complex serves as an E3-like ligase that conjugates phosphatidylethanolamine (PE) to microtubule-associated protein 1A/1B-light chain 3 (LC3) to form LC3-PE conjugate (LC3-II), which is recruited to autophagosomal membranes and is translocated to autolysosomal lumen where it is degraded. Atg5 is also reported to be involved in other cellular functions, including mitochondrial quality control following oxidative damage, negative regulation of the innate antiviral immune response, mitochondrial antiviral signaling protein (MAVS); lymphocyte development and proliferation; adipocyte differentiation, and apoptosis. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ye, X., et al. (2018). Front. Immunol. 9; 2334; Codogno, P., and Meijer, AJ. (2006). Nat. Cell Biol. 8(10); 1045-1047).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable