ZRB1401

Anti-Neuropeptide Y Antibody, clone 2B5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• 同義詞: NPY
• 分類程式碼代碼: 12352203
• NACRES: NA.43

屬性

• 生物源: rabbit
• 品質等級: 200
• 重組細胞: expressed in HEK 293 cells
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 2B5, recombinant monoclonal
• 描述: recombinant, expressed in HEK 293 cells
• 產品線: ZooMAb^® learn more
• 形狀: lyophilized
• 分子量: calculated mol wt 10.85 kDa
• 純化經由: using Protein A
• 物種活性: human
• 物種活性（以同源性預測）: mouse, rat
• 包裝: antibody small pack of 25 μL
• 環保替代產品特色: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• 技術: affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable
• 同型: IgG
• 表位序列: Unknown
• Protein ID登錄號: NP_000896
• UniProt登錄號: P01303
• 環保替代類別: , Aligned
• 運輸包裝: ambient
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NPY(4852)

描述

一般說明

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

特異性

Clone 2B5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects Neuropeptide Y.

免疫原

KLH-conjugated linear peptide corresponding to the mature Neuropeptide Y.

應用

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Neuropeptide Y in human cerebral cortex tissue sections.

Tested applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Neuropeptide Y in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound Neuropeptide Y with a KD of 5.8 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Immunohistochemistry (Paraffin) in human cerebral cortex tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected Neuropeptide Y in human cerebral cortex tissue sections.

標靶描述

Pro-neuropeptide Y (UniProt: P01303) is encoded by the NPY gene (Gene ID: 4852) in human. Neuropeptide Y is one of the most abundant peptides in the nervous system and is also found in some chromaffin cells of the adrenal medulla. In the brain it is present in the cortex, hippocampus, hindbrain and hypothalamus. It is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. It is shown to be one the most potent orexigenic peptides that stimulates food intake with a preferential effect on carbohydrate intake. It decreases latency to eat, increases motivation to eat and delays satiety by augmenting meal size. Its effects on feeding are mediated through the Y1 and Y5 receptors. Neuropeptide Y also acts as a vasoconstricting peptide that is released from sympathetic nerves in the heart and affects coronary artery constriction and myocardial contraction. It is also reported to potentiate the vasoconstrictor effects of other extracellular messengers, including a-adrenergic agonists and angiotensin II. It is also linked to inhibition of acetylcholine release from parasympathetic nerve endings in the heart. Neuropeptide Y is synthesized as a pre-propeptide with a signal peptide (aa 1-28) and a C-flanking peptide (aa 68-97) that are subsequently cleaved off to produce the active peptide (aa 29-64). This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Tan, CMJ., et al. (2018). Front. Physiol. 9; 1281; Beck, B. (2006). Philos. Trans. R. Soc. Lond. B Biol. Sci. 361(1471); 1159-1185).

外觀

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重構

Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.

儲存和穩定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律資訊

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable