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Z672548

高级PAP笔

Advanced PAP Pen
1 of 3 reviewers received a sample product or took part in a promotion

2 mm tip width

同義詞:

hydrophobic barrier pen, immunostaining wax pen, liquid blocker pap pen, water repellant barrier pen

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About This Item

分類程式碼代碼:
41181600
NACRES:
MA.01
暫時無法取得訂價和供貨情況

包裝

pack of 1 ea

製造商/商標名

Ted Pella 22311

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應用

用PAP笔在载玻片组织样本周围画一个疏水圈。通过将液体汇集成一个小液滴,避免浪费珍贵的试剂。不溶于乙醇、丙酮和水,但可以用二甲苯洗掉。

用于石蜡切片或用白蛋白或聚赖氨酸处理的载玻片。

數量

每支笔可以画600多个圈。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Aquatic Chronic 3 - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Ozone 1 - Repr. 1B - Skin Irrit. 2 - STOT RE 2 Inhalation - STOT SE 3

標靶器官

Central nervous system, Liver,Central nervous system, Respiratory system

水污染物質分類(WGK)

WGK 3

閃點(°F)

159.8 °F

閃點(°C)

71 °C


從最近期的版本中選擇一個:

分析證明 (COA)

Lot/Batch Number

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Gopinath Krishnamoorthy et al.
Disease models & mechanisms, 13(3) (2020-02-09)
Lactate dehydrogenase A (LDHA) mediates interconversion of pyruvate and lactate, and increased lactate turnover is exhibited by malignant and infected immune cells. Hypoxic lung granuloma in Mycobacterium tuberculosis-infected animals present elevated levels of Ldha and lactate. Such alterations in the

Questions

1–7 of 7 Questions  
  1. Will it hold in high temperatures? what is the highest temperature it can still be properly used in?

    1 answer
    1. This pen is stable at temperatures up to 120°C

      Helpful?

  2. Is there a hydrophobic pen available for immunofluorescence?

    1 answer
    1. Yes, there are hydrophobic pens available for immunofluorescence. The catalog numbers for the available pens are Z672548 and Z377821, and they are named Advanced PAP pen.

      Helpful?

  3. Will mounting a coverslip be a problem with the pap pen if my assay does not include a xylene step that removes this liquid barrier?

    1 answer
    1. This product is suitable for use with coverslip mounts so long as xylene is avoided.

      Helpful?

  4. Is this pen suitable to contain a drop of 3% H2O2 in methanol solution? Could the circle be erased by methanol?

    1 answer
    1. Yes, this product is resistant to most organic solvents and would not be affected by exposure to methanol. However, the barrier may be dissolved by xylene.

      Helpful?

  5. Hi, I have slide with 4x5 tissue spots of 5 mm size each. I like to draw hydrophobic barrier line to protect each tissue spots by drawing a column or row or grid. Therefore, i would require a fine tip Pap pen. Is 2 mm tip good or 1 mm will be better.

    1 answer
    1. The advanced PAP Pen is commonly used to create hydrophobic barriers on slides for various laboratory applications. It is often used to outline areas and draw boundaries to prevent mixing of reagents or solutions. Regarding the tip size, it depends on the specific requirements and preferences of the end-user. Both 2 mm and 1 mm tips can be suitable for creating hydrophobic barriers of the desired size on your slides. The choice between the two will depend on factors such as the precision needed by the end-user, the desired thickness or width of the barrier, and the personal experience and comfort with using different tip sizes of the end-user's.

      If a finer and more precise line is required, a 1 mm tip may be preferable. However, if a slightly wider or more visible barrier line is needed, the 2 mm tip can be suitable.

      Helpful?

  6. How much volume does the pen contain? I couldn't find it in the specification sheet. Thanks,

    1 answer
    1. This pen contains approximately 7.5 mL of liquid. The volume is sufficient to place more than 600 circles.

      Helpful?

  7. Does this pen marking autofluorese?

    1 answer
    1. This pen is suitable for use with fluorescent antibodies. It is not specifically tested for autofluorescence; no reports or complaints of this occurring have been filed. This PAP Pen Liquid Blocker, is a hydrophobic slide marker for staining procedures, creates a visible hydrophobic barrier that provides proper surface tension to hold reagents within a targeted area when applied around tissue sections or smears on a microscopic slide. This surface tension ensures the amount of reagent needed for sufficient reaction is reduced and retained for complete tissue section coverage. Multiple specimens can be separated by drawn circles or lines applied to the same slide.

      PAP Pen Liquid Blockers contain a unique formulation that is water repellent, insoluble in alcohol and acetone, soluble in xylene and temperature resistant up to 120°C.

      Helpful?

Reviews

1 of 3 reviewers received a sample product or took part in a promotion

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  1. Portugal
    • Review 1
    • Votes 0
    4 out of 5 stars.

    Useful

    It is so useful in IHC because it allows you to use less ab

    Helpful?

  2. 2 Ratings-Only Reviews

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