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X2753

Sigma-Aldrich

木聚糖酶

greener alternative

powder, ≥2500 units/g, recombinant, expressed in Aspergillus oryzae

同義詞:

Pentopan Mono BG®

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About This Item

CAS號碼:
37278-89-0
EC號碼:
253-439-7
MDL號碼:
MFCD00132594
分類程式碼代碼:
12352204
NACRES:
NA.54
暫時無法取得訂價和供貨情況

重組細胞

expressed in Aspergillus oryzae

形狀

powder

比活性

≥2500 units/g

環保替代產品特色

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

環保替代類別

Enabling,

儲存溫度

2-8°C

相關類別

一般說明

从一种转基因米曲霉微生物的深度发酵生产的疏棉状嗜热丝孢菌中纯化的内切-β−（1→4）-木聚糖酶
我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。当用于纤维素乙醇研究时，该产品经优化可提高能效和防止浪费。有关更多信息，请参见 生物文档中的文章 和 替代能源研究用酶
木聚糖酶催化木聚糖降解为低聚木糖和D-木糖。它还可以分解半纤维素。木聚糖酶用于生产生物燃料，例如乙醇和木糖醇。它提高了面包和果汁的质量。[1]木聚糖酶用于生物漂白过程并减少环境污染。[2]
研究表明，木聚糖酶基因中发现的信号序列对大肠杆菌中蛋白质的产生至关重要。该序列的丢失导致表达显着降低。 [3]

應用

木聚糖酶已用于：
  • 分离半纤维素和生产低聚糖[4]
  • 粘度测定[5]
  • 比较玉米胚芽水酶法提取玉米油的商业酶[6]
  • 木聚糖的水解降解[7]

其他說明

有关复合碳水化合物分析酶的更多信息，请见www.sigma-aldrich.com/enzymeexplorer

法律資訊

Novozyme 公司的产品
Pentopan is a registered trademark of Novozymes Corp.

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H334

防範說明

P261 - P284 - P501

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000424723
0000424722
0000424722
0000424723
0000374140

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

Xylan-binding xylanase Xyl30 from Streptomyces avermitilis: cloning, characterization, and overproduction in solid-state fermentation
Hernandez A, et al.
International Microbiology : The Official Journal of the Spanish Society for Microbiology (2008)
Microbial xylanases and their industrial application in pulp and paper biobleaching: a review
Walia A, et al.
3 Biotech, 7(1), 11-11 (2017)
Svini Dileepa Marasinghe et al.
Microbial cell factories, 20(1), 129-129 (2021-07-10)
Xylanase-containing enzyme cocktails are used on an industrial scale to convert xylan into value-added products, as they hydrolyse the β-1,4-glycosidic linkages between xylopyranosyl residues. In the present study, we focused on xynS1, the glycoside hydrolase (GH) 11 xylanase gene derived
XYLANASES AND THEIR INDUSTRIAL APPLICATIONS: A REVIEW
Kumar D, et al.
Biochemical and Cellular Archives, 17(1), 353-360 (2017)
Gautam Anand et al.
International journal of molecular sciences, 22(8) (2021-05-01)
Plants lack a circulating adaptive immune system to protect themselves against pathogens. Therefore, they have evolved an innate immune system based upon complicated and efficient defense mechanisms, either constitutive or inducible. Plant defense responses are triggered by elicitors such as
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Questions

1–7 of 7 Questions  

  1. Will this enzyme in buffer be deactivated completely at 100°C boiling for 10 mins towards end of protocol or it needs acid treatment strictly ?

    1 answer

    1. We have not tested boiling this enzyme at the end of the protocol, we have only used acid.

      Helpful?

  2. Is this enzyme powder soluble in water ? seems like particle dispersing in buffer at Ph 4.8 ?

    1 answer

    1. The active components of xylanase X2753 are readily soluble in water at concentrations that occur in normal usage. However, water solutions will be turbid because of the wheat flour used for standardization of the enzyme.

      Helpful?

  3. What does >2500U/g imply. What is range of enzyme activity for this enzyme under your protocols kindly inform.

    1 answer

    1. Each Lot is guaranteed to have a specific activity greater than or equal to 2500 Units/g. There is no maximal value specified.
      The calculated specific activity for each lot will be printed on the Certificate of Analysis found here:
      https://www.sigmaaldrich.com/product/sigma/x2753#product-documentation

      Helpful?

  4. What is the optimal pH, buffer, temperature and concentration of this product ?

    1 answer

    1. This is a product of Novozymes Corp. The manufacturer tests this material according to the following protocol and conditions:
      Temperature - 50°C
      Buffer - 0.1M Phosphate Buffer, pH 6
      Enzyme concentration - 0.007 - 0.057 units/mL
      Substrate - 0.45%w/v Azo-Wheat arabinxylan in 0.1 M Phosphate Buffer, pH 6
      Reaction - 0.100 mL enzyme solution added to 0.900 mL substrate solution
      Incubation time - 30 minutes in a water bath at 50°C
      Stop Reaction - add 5 mL of Stop Reagent: 6.66 mL 2 N HCl in 1000mL absolute Ethanol
      Incubation - 15 - 60 minutes at ambient temperature
      Centrifuge at 4000 rmp for 15 minutes
      Detection - 585 nm (manual) within 20 minutes of centrifugation

      Helpful?

  5. We have Sigma X2753 Xylanase We need to treat our sample with this. Kindly suggest a protocol at which this product will show its optimum activity.

    1 answer

    1. There is not a protocol offered specifically for this product. However, the peer-reviewed articles may be helpful:
      https://www.sigmaaldrich.com/search/x2753?focus=papers&page=1&perpage=30&sort=relevance&term=x2753&type=citation_search

      Helpful?

  6. What is the density of Product X2753, Cellulase from Aspergillus oryzae?

    1 answer

    1. We have not determined the density of this product.

      Helpful?

  7. What is the optimum pH for Cellulase from Aspergillus oryzae., Product X2753?

    1 answer

    1. Product X2753, Xylanase, has activity in the pH range of 4-6, and tolerance with temperatures up to 75 °C. The following article may be helpful: https://www.sciencedirect.com/science/article/pii/S138917230670603X#aep-section-id10

      Helpful?

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