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重要文件

T7575

Sigma-Aldrich

蛋白质组学级胰蛋白酶单品

同義詞:

Proteomics grade, Trypsin, TPCK treated

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About This Item

分類程式碼代碼:
12352204
NACRES:
NA.56
暫時無法取得訂價和供貨情況

等級

Proteomics Grade

形狀

ready-to-use solution

比活性

≥10,000 BAEE units/mg protein

分子量

average mol wt 23.29 kDa

最適pH

~8.0

儲存溫度

2-8°C

一般說明

采用方便的一次性1 μg包装,体验蛋白质组学级胰蛋白酶的所有优点。

蛋白质组学级胰蛋白酶单品具有出色的蛋白水解效率,可产生更多的胰蛋白酶肽,从而为您感兴趣的肽提供更大的序列覆盖率。由于干扰性自溶峰及其模糊加合物的数量减少,从而大大简化了质谱分析。目前已经从猪胰腺分离出大量蛋白质组学级胰蛋白酶,使其能够在Arg和Lys残基的羧酸侧实现精准切割。通过对酶进行彻底的还原性甲基化处理,能够最大限度地减少自溶,并且已通过TPCK处理淬灭了胰凝乳蛋白酶活性。[1]通过亲和层析以及稀酸干燥等进一步纯化步骤,获得高纯度的高比活性胰蛋白酶,特别符合蛋白质组学研究的苛刻标准,并且专用于溶液或凝胶类消化物。

應用

蛋白质组学级胰蛋白酶单品已被用于蛋白质消化,以通过MALDI-TOF MS(基质辅助激光解吸电离-飞行时间质谱法)分析产生的肽。[2][3]它已被用于蛋白质消化,以通过LC-MS(液相色谱-质谱法)/MS分析产生的肽。[4][5]

特點和優勢

  • 简单方便,一次性1 μg用量
  • 减少重复加样
  • 还原性甲基化以减少自溶活性
  • TPCK处理以消除胰凝乳蛋白活性
  • 高度纯化

套裝中的組件也可單獨購買

產品號碼
描述
SDS

  • Trypsin Solubilization Reagent

相關產品

產品號碼
描述
訂價

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

3 - Flammable liquids


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存取文件庫

Melt With This Kiss: Paralyzing and Liquefying Venom of The Assassin Bug Pristhesancus plagipennis (Hemiptera: Reduviidae).
Walker AA, et al.
Molecular and Cellular Proteomics, 16, 552-552 (2017)
Zahraa I Khamis et al.
Journal of Cancer, 1, 70-79 (2010-09-16)
In situ neoplastic prostate cells are not lethal unless they become invasive and metastatic. For cells to become invasive, the prostate gland must undergo degradation of the basement membrane and disruption of the basal cell layer underneath the luminal epithelia.
Tiziana Cabras et al.
Molecular & cellular proteomics : MCP, 9(10), 2099-2108 (2010-06-30)
The acidic soluble fraction of whole saliva of type 1 diabetic children was analyzed by reversed phase (RP)(1)-HPLC-ESI-MS and compared with that of sex- and age-matched control subjects. Salivary acidic proline-rich phosphoproteins (aPRP), histatins, α-defensins, salivary cystatins, statherin, proline-rich peptide
Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry.
Tripkovic T, et al.
Talanta, 113, 49-61 (2013)
David J Gonzalez et al.
The Journal of biological chemistry, 285(36), 28220-28228 (2010-06-29)
Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes

Questions

  1. How many 1 µg samples are included in the proteomics grade Trypsin kit (T7575-1KT)?

    1 answer
    1. The proteomics grade Trypsin kit (T7575-1KT) includes 96 individual 1 µg samples, each provided in a single vial.

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