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Merck
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重要文件

SRP6495

Sigma-Aldrich

IGF 1R/CD221 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

同義詞:

IGF-1R, IGFIR, IGFR, IGFⅠR, Insulin-like Growth Factor 1 (IGF-1) Receptor, JTK13, MGC142170, MGC142172, MGC18216

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

生物源

human

重組細胞

expressed in HEK 293 cells

籤條

6-His tagged (C-terminus)

化驗

≥95% (SDS-PAGE)

形狀

lyophilized

分子量

β subunit calculated mol wt 23 kDa
A subunit calculated mol wt 80 kDa
single-chain calculated mol wt 104 kDa
35 kDa
observed mol wt 120 kDa by SDS-PAGE (DTT-reduced.)
observed mol wt 80 kDa

包裝

pkg of 10 and 50 μg

雜質

<1 EU/μg endotoxin (LAL test)

UniProt登錄號

運輸包裝

wet ice

儲存溫度

−20°C

基因資訊

human ... IGF1R(3480)

一般說明

The Insulin-like Growth Factor 1 Receptor (IGF1R)is also known as CD221, JTK13, and is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF1R is made up of two α subunits and two β subunits. Both the α and β subunits are synthesized from a single mRNA precursor. The precursor is then glycosylated, proteolytically cleaved, and crosslinked by cysteine bonds to form a functional transmembrane αβ chain. The α chains are located extracellularly while the β subunit spans the membrane and are responsible for intracellular signal transduction upon ligand stimulation. IGF1R have a binding site for ATP, which is used to provide the phosphates for autophosphorylation. There is a 60% homology between IGF1R and the insulin receptor. In response to ligand binding, the α chains induce the tyrosine autophosphorylation of the β chains. This event triggers a cascade of intracellular signaling that, while somewhat cell type specific, often promotes cell survival and cell proliferation.

外觀

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

重構

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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