推薦產品
重組細胞
expressed in baculovirus infected Sf9 cells
產品線
PRECISIO® Kinase
化驗
≥70% (SDS-PAGE)
形狀
buffered aqueous glycerol solution
比活性
157-213 nmol/min·mg
分子量
~59 kDa (CDK5)
~60 kDa (p35)
技術
activity assay: suitable
溶解度
soluble
water: soluble
運輸包裝
dry ice
儲存溫度
−70°C
基因資訊
human ... CDK5(1020) , CDK5R1(8851)
一般說明
Research area: Apoptosis
Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.
Cdk5 is a proline-directed serine/threonine protein kinase that belongs to the family of cyclin-dependent kinases (CDKs). Active CDK5, also known as neuronal cdc2-like kinase is a heterodimer of CDK5 and is essential for its kinase activity. They are found abundantly in the mammalian brain.
應用
CDK5 has been used as a component of a buffer during the measurement of the in vitro phosphorylation of peroxisome proliferator-activated receptor gamma (PPARγ).
生化／生理作用
CDK5 is a neuron-specific kinase that plays a vital role in the development, functioning, and diseases of the central nervous system. Its optimum functioning is highly essential for proper neuronal migration, differentiation, axonal elongation, and synaptic function. CDK5 modulates neurite outgrowth, and axonal growth by interacting with several substrates like N- cadherin, Rap Guanine Nucleotide Exchange Factor 2(RapGEF2), focal adhesion kinase (FAK), p21 activating kinase 1, serotonin 6 receptor and Ras-related protein 8. CDK5 also plays a key role in the formation of the synapse, its maintenance, and communication. It is involved in the formation and retraction of the dendritic spines. It also regulates neuron secretion at the synapse by phosphorylating key mediators such as Synaspsin1. In addition, CDK5, present in the synaptic membrane phosphorylates the erythroblastic leukemia viral oncogene homolog B receptor (ErbB), resulting in increased acetylcholine receptor transcription. Lastly, CDK5 is also involved in the regulation of various other cellular processes like cell cycle, cell survival, apoptosis, gene regulation, etc.
外觀
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
準備報告
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
法律資訊
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Davide Capelli et al.
Biomolecules, 13(4) (2023-05-16)
PPARγ represents a key target for the treatment of type 2 diabetes and metabolic syndrome. To avoid serious adverse effects related to the PPARγ agonism profile of traditional antidiabetic drugs, a new opportunity is represented by the development of molecules
Franck Peiretti et al.
Journal of medicinal chemistry, 63(21), 13124-13139 (2020-11-04)
A proprietary library of novel N-aryl-substituted amino acid derivatives bearing a hydroxamate head group allowed the identification of compound 3a that possesses weak proadipogenic and peroxisome proliferator-activated receptor γ (PPARγ) activating properties. The systematic optimization of 3a, in order to
D Tang et al.
Progress in cell cycle research, 2, 205-216 (1996-01-01)
While cyclin-dependent kinase 5 (Cdk5) is widely distributed in mammalian tissues and in cultured cell lines, Cdk5-associated kinase activity has been demonstrated only in mammalian brains. An active form of Cdk5, called neuronal cdc2-like kinase (Nclk) has been purified from
R Dhavan et al.
Nature reviews. Molecular cell biology, 2(10), 749-759 (2001-10-05)
Since it was identified a decade ago, cyclin-dependent kinase 5 (CDK5) has emerged as a crucial regulator of neuronal migration in the developing central nervous system. CDK5 phosphorylates a diverse list of substrates, implicating it in the regulation of a
Antonio Laghezza et al.
Journal of medicinal chemistry, 61(18), 8282-8298 (2018-09-11)
A new series of derivatives of the PPARα/γ dual agonist 1 allowed us to identify the ligand ( S)-6 as a potent partial agonist of both PPARα and γ subtypes. X-ray studies in PPARγ revealed two different binding modes of
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