SRP3141

Persephin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 同義詞: PSP, PSPN
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 分子量: 10.3 kDa
• 包裝: pkg of 20 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: O60542
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... PSPN(5623)

描述

一般說明

Persephin is a disulfide-linked homodimer neurotrophic factor structurally related to glial cell line-derived neurotrophic factor (GDNF), artemin, and neurturin. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Persephin signals through a multicomponent receptor system, composed of RET and one of four GFRa (a1-a4) receptors. The GFRa4 was first identified in chicken and was later shown to be the preferential binding subunit for Persephin. The gene coding for persephin is localized on human chromosome 19p13.3.[1] Recombinant human persephin is a disulfide-linked homodimer, composed of two 10.3kDa polypeptide chains (192 total amino acid residues). Each chain contains seven conserved cysteine residues, one of which (Cys 63) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.

生化／生理作用

Persephin stimulates the survival of ventral midbrain dopaminergic neurons and motor neurons[2] after sciatic nerve axotomy, and like GNDF, promotes ureteric bud branching. However, persephin does not support the survival of peripheral neurons.[3]

序列

ALSGPCQLWS LTLSVAELGL GYASEEKVIF RYCAGSCPRG ARTQHGLALA RLQGQGRAHG GPCCRPTRYT DVAFLDDRHR WQRLPQLSAA ACGCGG

外觀

Lyophilized from 0.3x PBS, pH 7.2.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer and store in working aliquots at -20°C to -80°C.

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable