SRP3138

PDGF-BB human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 同義詞: Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF), Platelet-Derived Growth Factor-BB
• 分類程式碼代碼: 12352202
• eCl@ss: 32190102
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形狀: lyophilized
• 效力: 1.0-3.0 ng/mL ED₅₀
• 分子量: 24.3 kDa
• 包裝: pkg of 10 μg
• 技術: cell culture | mammalian: suitable
• 雜質: <0.1 EU/μg endotoxin, tested
• 顏色: white to off-white
• UniProt登錄號: P01127
• 運輸包裝: wet ice
• 儲存溫度: −20°C
• 基因資訊: human ... PDGFB(5155)

描述

一般說明

PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. The gene PDGF-B is mapped to human chromosome 22q13.1. Recombinant human PDGF-BB is a 24.3kDa disulfide-linked homodimer of two B chains (218 total amino acids).

應用

PDGF-BB human has been used in the cell viability assay. It has been used as a stimulator in PDGFRα (platelet-derived growth factor receptor α)-positive HTPCs (human testicular peritubular cells).

生化／生理作用

The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. PDGF-B along with PDGFR-β participates in vascular maturation. It brings smooth muscle cells and pericytes to growing vessels. Heterozygous mutation in PDGF-B is associated with primary familial brain calcification.

序列

SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE TVAAARPVT

外觀

Lyophilized from 10 mM Acetic Acid.

重構

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H315,H319
• 防範說明: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• 危險分類: Eye Irrit. 2 - Skin Irrit. 2
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable