The product SRP3118 does not need activation with APMA since it is not a pro-MMP; it is expressed as the mature sequence.
推薦產品
生物源
human
重組細胞
expressed in E. coli
化驗
≥98% (HPLC)
≥98% (SDS-PAGE)
形狀
lyophilized
分子量
62.0 kDa
包裝
pkg of 10 μg
技術
cell culture | mammalian: suitable
雜質
<0.1 EU/μg endotoxin, tested
顏色
white to off-white
UniProt登錄號
運輸包裝
wet ice
儲存溫度
−20°C
基因資訊
human ... MMP2(4313)
一般說明
生化／生理作用
序列
MYNFFPRKPK WDKNQITYRI IGYTPDLDPE TVDDAFARAF QVWSDVTPLR FSRIHDGEAD IMINFGRWEH GDGYPFDGKD GLLAHAFAPG TGVGGDSHFD DDELWTLGEG QVVRVKYGNA DGEYCKFPFL FNGKEYNSCT DTGRSDGFLW CSTTYNFEKD GKYGFCPHEA LFTMGGNAEG QPCKFPFRFQ GTSYDSCTTE GRTDGYRWCG TTEDYDRDKK YGFCPETAMS TVGGNSEGAP CVFPFTFLGN KYESCTSAGR SDGKMWCATT ANYDDDRKWG FCPDQGYSLF LVAAHEFGHA MGLEHSQDPG ALMAPIYTYT KNFRLSQDDI KGIQELYGAS PDIDLGTGPT PTLGPVTPEI CKQDIVFDGI AQIRGEIFFF KDRFIWRTVT PRDKPMGPLL VATFWPELPE KIDAVYEAPQ EEKAVFFAGN EYWIYSASTL ERGYPKPLTS LGLPPDVQRV DAAFNWSKNK KTYIFAGDKF WRYNEVKKKM DPGFPKLIAD AWNAIPDNLD AVVDLQGGGH SYFFKGAYYL KLENQSLKSV KFGSIKSDWL GC
外觀
自10 mM磷酸钠、pH 7.5 + 0.1 mM氯化钙冻干。
重構
打开前离心小瓶。在水中复原至0.5-1.0 mg/ml的浓度。不要涡旋。该溶液可在2-8°C条件下储存长达1周的时间。对于长期储存，建议在含有载体蛋白的缓冲液中进一步稀释（示例0.1% BSA），并在-20°C至-80°C条件下储存在工作等分试样中。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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Can you confirm if this product is an activated form of MMP-2, or does it require activation with APMA?
1 answer-
Helpful?
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What is the test method for Product No. SRP3118-10UG, MMP-2 Recombinant Human?
1 answer-
The test method for Product No. SRP3118-10UG, MMP-2 Recombinant Human involves measuring the MMP-2 human bioactivity by its ability to cleave a chromogenic peptide MMP-2 substrate at room temperature. This was done at an MMP-2 concentration of 2.5 ug/ml, achieving 50% cleavage at an incubation time of approximately 25 minutes. The protocol specifies using a kit supplied by AnaSpec (Catolog#72095), which includes diluting the colorimetric substrate, reconstituting the MMP-2 sample with water, mixing the MMP-2 and substrate solution, and taking readings at 412nm every 10 minutes for about 90 minutes or until a steady reading is obtained.
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