SRP0213

PTP1B (1-321)/PTPN1 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 同義詞: PTPN1, Protein tyrosine phosphatase, non-receptor type 1
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: mouse
• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 63.3 kDa
• 包裝: pkg of 20 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_011201.3
• UniProt登錄號: P35821
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PTPN1(5770)

描述

一般說明

Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

應用

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

單位定義

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

外觀

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.