SRP0210

PRL-3 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• 同義詞: PRL-3, PTP4A3, Protein tyrosine phosphatase type IVA, member 3, Protein-tyrosine phosphatase of regenerating liver 3
• 分類程式碼代碼: 51111800
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in E. coli
• 化驗: ≥90% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 19.5 kDa
• 包裝: pkg of 50 μg
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBI登錄號: NM_032611
• UniProt登錄號: O75365
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PTP4A3(11156)

描述

一般說明

Human PRL 3 (GenBank Accession No. NM_032611), (amino acids 2-173) with N-terminal HIS tag, MW = 19.5 kDa, expressed in an E Coli expression system.

應用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

單位定義

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

外觀

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.