SRP0145

PRMT5/MEP50 Active human

recombinant, expressed in FreeStyle^™ 293-F cells, ≥60% (SDS-PAGE)

• 同義詞: Arginine methyltransferase 5, HMT1 hnRNP methyltransferase-like 5(HRMT1L5), ICln-binding protein (IBP72) 72 kDa, Jak-binding protein 1 (JBP1), shk1 kinase-binding protein 1 homolog (SKB1)
• 分類程式碼代碼: 12352200
• NACRES: NA.32

屬性

• 生物源: human
• 重組細胞: expressed in FreeStyle^™ 293-F cells
• 化驗: ≥60% (SDS-PAGE)
• 形狀: aqueous solution
• 分子量: 37.5 kDa (MEP50); 73 kDa (PRMT5)
• 包裝: pkg of 20 μg
• 製造商／商標名: Sigma-Aldrich
• 儲存條件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• 技術: inhibition assay: suitable
• 溶解度: water: soluble
• NCBI登錄號: NM_006109 (PRMT5); NM_024102 (MEP50)
• UniProt登錄號: O14744
• 應用: life science and biopharma
• 運輸包裝: dry ice
• 儲存溫度: −70°C
• 基因資訊: human ... PRMT5(10419)

描述

一般說明

Research area: Cell signaling. Protein arginine methyltransferase 5 (PRMT5) and WD repeat-containing protein (WDR77) or methylosome protein- 50 (MEP-50) are part of the methylosome. PRMT5 is a type II PRMT enzyme. The gene encoding it is localized to human chromosome 14q11.2.Complex of human PRMT5 (GenBank Accession No. NM_006109), amino acid 2-end, with N-terminal FLAG^®-tag, MW=73 kDa, and human MEP50 (GenBank Accession No. NM_024102), amino acid 2-end, with N-terminal His tag, MW=37.5 kDa, expressed in FreeStyle 293-F cells.

應用

Recombinant PRMT5/MEP50 has been used in the detection of arginine methylation in in vitro methyltransferase assay.

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

生化／生理作用

Methylosome protein 50 (MEP50) binds to the oncogenic protein arginine methyltransferase 5 (PRMT5) and forms a complex called methylosome. This complex is involved in mono- and symmetric dimethylation of arginine, which is necessary for various biological processes. MEP50 acts as a coactivator for androgen receptor (AR) and estrogen receptor (ER). PRMT5 also methylates histones and other proteins involved in genome organization, transcription, stem cells, primordial germ cells, differentiation, the cell cycle, and spliceosome assembly. The two proteins have been associated with cancer tumorigenesis and are marked as potential oncogenes.A boost in MEP50 levels is observed in several human cancers such asastrocytoma, ovarian cancer, lung cancer, glioblastoma, breast cancer,testicular tumor, and prostate cancer.

單位定義

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

外觀

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 80 μg/ml FLAG^® peptide, and 20% glycerol.

準備報告

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

法律資訊

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FreeStyle is a trademark of Invitrogen Corp.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable