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P9290

Sigma-Aldrich

蛋白酶K-琼脂糖 来源于林伯氏白色念球菌

lyophilized powder

同義詞:

蛋白酶K-琼脂糖

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About This Item

MDL號碼:
分類程式碼代碼:
41106500
NACRES:
NA.56

生物源

microbial (T.album
T. ALBUM)

形狀

lyophilized powder

標籤範圍

30-140 units per g solid

技術

DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable

膨潤

1 g swells to ~5 mL

儲存溫度

−20°C

應用

蛋白酶K-琼脂糖被用于分离蛋白质和核酸、修饰酶和固定酶。蛋白酶K-琼脂糖已被用于研究家犬的肺炎克雷伯菌(肺炎克雷伯菌)和多房棘球绦虫(Echinococcus multilocularis)感染。

生化/生理作用

通过将蛋白酶K固定在不溶性基质上,如琼脂糖,可将其用于更多应用中。琼脂糖在大多数系统中都是惰性的,并且每单位重量具有很高的活性。利用这种反应性树脂,通过酶与底物在合适介质中的瞬时接触,能够迅速催化酶反应。反应可以在间歇桨液反应器中进行,甚至可以在小柱中进行。无论是哪种情况,不溶性酶都不会污染反应混合物,可以很容易地将其从间歇桨液中滤出或留在色谱柱中。

特點和優勢

•基质载体上的可回收蛋白酶 K。
•可以利用旋转柱处理或分批处理。

單位定義

在pH 7.5、37℃条件下,一单位每分钟可水解尿素变性血红蛋白并产生相当于1.0 μ摩尔 (181 μg) 酪氨酸的显色(使用Folin-Ciocalteu试剂显色)。

外觀

用乳糖稳定化的冻干粉

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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