跳轉至內容
Merck
全部照片(1)

重要文件

檢視所有文件

SHPMLIBR

Sigma-Aldrich

MISSION® LentiPlex® 完整小鼠混合shRNA库

For Rapid, Convenient Genome-wide shRNA Screens

登入查看組織和合約定價
全部照片(1)

About This Item

分類程式碼代碼:
41105904
NACRES:
NA.51
暫時無法取得訂價和供貨情況

產品線

MISSION®

濃度

≥5x108 VP/ml (via p24 assay)

技術

capture ELISA: 5 x 108 TU/mL using p24

運輸包裝

dry ice

儲存溫度

−70°C

相關類別

一般說明

MISSION LentiPlex 混合文库结合了MISSION TRC shRNA系列的强大能力与Sigma的慢病毒制造专业知识，可在您的工作台上实现全基因组RNAi筛选。LentiPlex混合系统可对非分裂和原代细胞系提供增强递送和长期基因沉默效果，可带来无限的探索潜力。这种方便的形式允许任何研究人员使用快速全基因组RNAi筛选方法，仅需极少的试剂、时间或资金设备投入。

MISSION LentiPlex 完整小鼠shRNA混合文库是使用专有工艺生产的全基因组慢病毒库。该文库包括110,000多个shRNA构建体，来自靶向21,000个小鼠基因的TRC集合。在产品发布之前，每个文库都要测试shRNA表达，以确保可靠的文库覆盖范围。文库以即用型慢病毒形式提供，通过p24检测，滴度至少为5 x 108 TU/ml，并预先分为15个子库，每个子库约有5,500 - 8000个shRNA构建体。另外提供用于下游靶标识别的扩增和测序引物。

有关更多信息，包括样品数据和用途，请访问LentiPlex技术详细信息页面。

其他說明

MISSION LentiPlex 小鼠混合shRNA文库包括货号SHPM01、SHPM15、SHPM2和SHPMLIBR的产品。请参阅表1了解每个文库相应的shRNA克隆库覆盖范围、靶标基因和子库数。

另外提供用于下游比中识别的扩增和测序引物。

shRNA库反卷积
Sigma反卷积服务可让您轻松识别影响混合shRNA筛选的基因。

  • 新一代克隆测序可精确测定混合样品中的单个克隆次数。
  • 确保混合shRNA筛选实现全面、可重现的结果
  • 稳健统计和信息丰富的数据

联系当地Sigma销售代表，了解更多信息或提交MISSION RNAi咨询

推薦產品

在我们的MISSION shRNA门户网站中浏览其他MISSION shRNA基因家族集

法律資訊

慢病毒许可协议
LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

相關產品

產品號碼
描述
訂價

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如果您需要協助，請聯絡 客戶支援

已經擁有該產品？

您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

客戶也查看了

Slide 1 of 1

1 of 1

MISSION® shRNA Bacterial Stock

Sigma-Aldrich

SHCLNG

MISSION® shRNA

Christine Karlsson et al.
Methods in molecular biology (Clifton, N.J.), 650, 29-43 (2010-08-06)
Identifying the genes and pathways that regulate self-renewal and differentiation in somatic stem cells is a central goal in stem cell and cancer biology. Here, we describe a method for RNAi-based screens in primary human hematopoietic stem and progenitor cells.
Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.
Lin, Yu-yi, et al.
Nature (2012)
Richard Possemato et al.
Nature, 476(7360), 346-350 (2011-07-16)
Cancer cells adapt their metabolic processes to drive macromolecular biosynthesis for rapid cell growth and proliferation. RNA interference (RNAi)-based loss-of-function screening has proven powerful for the identification of new and interesting cancer targets, and recent studies have used this technology

文章

shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

shRNA 製程與圖表

當使用慢病毒載體傳遞 shRNA 時，編碼 shRNA 的序列會整合到基因組中，而基因敲除的效果會傳給子細胞，繼續基因沉默。

Safe Lentivirus Handling

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

查看全部

條款

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

慢病毒的 FACS 滴定協議

FACS 根據光散射和螢光來分類細胞，以進行客觀的細胞分析。

相關內容

CRISPR Lentiviral Screening: Antibiotic Selection

You are not alone designing successful CRISPR, RNAi, and ORF experiments. We were the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

Safe Lentivirus Handling

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

Questions

Reviews

No rating value

Active Filters

我們的科學家團隊在所有研究領域都有豐富的經驗，包括生命科學、材料科學、化學合成、色譜、分析等.

聯絡技術服務