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Merck
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重要文件

SAB5700611

Sigma-Aldrich

Anti-Glial Fibrillary Acidic Protein Antibody

rabbit polyclonal

同義詞:

ALXDRD, GFAP

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.41
暫時無法取得訂價和供貨情況

產品名稱

Anti-GFAP antibody produced in rabbit,

生物源

rabbit

品質等級

100

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

49
 50

物種活性

human, mouse, rat

濃度

2.32 mg/ml

技術

immunofluorescence: 1:50-1:200
western blot: 1:500-1:2000

UniProt登錄號

P14136

運輸包裝

wet ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... GFAP(2670)

一般說明

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

免疫原

A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).

外觀

PBS with 0.02% sodium azide,50% glycerol,pH7.3.

儲存和穩定性

Store at -20℃. Avoid freeze / thaw cycles.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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分析證明 (COA)

Lot/Batch Number
3560492113
3560492111

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Questions

  1. こんにちは、マウスのアストロサイトにおけるGFAP含有量を測定したいと考えています。免疫蛍光法を使用する予定で、現在Anti-GFAP （SAB5700611）とAnti-Rabbit IgG (H+L)（SAB4600045）の2つの抗体を選んでいます。これらの抗体は理論的には相互に結合できますか？ また、これら2つの抗体の推奨濃度についても教えていただけると嬉しいです。 よろしくお願いいたします。

    1 answer

    1. Anti-GFAP (SAB5700611) is a primary antibody targeting the Glial fibrillary acidic protein found in astrocytes. When used in conjunction with Anti-Rabbit IgG (H+L) (SAB4600045) in an experiment, e.g., immunofluorescence or western blot, the goat-produced Anti-Rabbit IgG (H+L) secondary antibody (SAB4600045) would bind to the rabbit-produced Anti-GFAP primary antibody. This is because the secondary antibody is specific for rabbit immunoglobulins, and the primary antibody is produced in rabbits, thus containing rabbit IgG.
      Regarding the working concentrations, see the dilutions factors for each item below. Please note that this information is also listed in the Properties section of the product page.
      SAB5700611
      immunofluorescence: 1:50-1:200
      western blot: 1:500-1:2000

      SAB4600045
      flow cytometry: 1-10 μg/mL
      immunocytochemistry: suitable
      immunohistochemistry: suitable
      indirect immunofluorescence: 1-10 μg/mL

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