SAB4702127

Monoclonal Anti-CD22 PE-Cyanine7 produced in mouse

• 同義詞: SIGLEC-2, SIGLEC2
• 分類程式碼代碼: 12352203

屬性

• 共軛: PE-Cy7 conjugate
• 品質等級: 100
• 抗體產品種類: primary antibodies
• 無性繁殖: S-HCL-1, monoclonal
• 形狀: buffered aqueous solution
• 物種活性: human
• 技術: flow cytometry: suitable
• 同型: IgG2bκ
• UniProt登錄號: P20273
• 儲存溫度: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CD22(933)

描述

一般說明

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

特異性

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

免疫原

Whole hairy cell leukemia cells and membrane preparation

外觀

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

準備報告

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

儲存和穩定性

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

其他說明

The antibody solution should be gently mixed before use.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable