SAB4200786

Anti-Human IgG1 antibody, Mouse monoclonal

clone 8C/6-39, purified from hybridoma cell culture

• 同義詞: Anti-Human immunoglobulin G1
• 分類程式碼代碼: 12352203
• NACRES: NA.46

屬性

• 生物源: mouse
• 品質等級: 200
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: 8C/6-39, monoclonal
• 形狀: buffered aqueous solution
• 物種活性: human
• 濃度: ~1 mg/mL
• 技術: indirect ELISA: 3-6 μg/mL using Human IgG1 myeloma protein for coating.
• 同型: IgG2a
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified

描述

一般說明

Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. IgG1 and IgG3 are the only subclasses capable of adherence to mononuclear phagocytes and are recognized readily by the Fc receptors on various reticulo-endothelial cells while IgG2 and IgG4 are far less efficient. The amount of the different IgG subclasses present in the bloodstream varies with age. For example, IgG1 and IgG3 reach normal adult levels by 5-7 years of age while IgG2 and IgG4 levels raise more slowly, reaching adult levels at about 10 years of age. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, a disproportionate elevation of IgG1 has been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and thus may assist in the diagnosis of various disorders.

免疫原

The Fc fragment of a human IgG1 myeloma protein.

應用

Indirect ELISA: a working concentration of 3-6 μg/mL is recommended using 10 μg/mL Human IgG1 myeloma protein for coating.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15mM sodium azide.

其他說明

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable