SAB4200594

Anti-APC (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 同義詞: Anti-Adenomatous polyposis coli protein, Anti-BTPS2, Anti-DP2, Anti-DP2.5, Anti-DP3, Anti-FAP, Anti-GS
• 分類程式碼代碼: 12352203
• NACRES: NA.41
• 共軛: unconjugated
• application: IF; IHC
• 無性繁殖: polyclonal
• 物種活性: human
• citations: 4
• 技術: immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells.; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum.; indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells.

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~300 kDa
• 物種活性: human
• 濃度: ~1.0 mg/mL
• 技術: immunoblotting: 3-6 μg/mL using lysates of HeLa or SW620 cells.; immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon or duodenum.; indirect immunofluorescence: 2-4 μg/mL using Caco-2 cells.
• UniProt登錄號: P25054
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... APC(324)

描述

一般說明

Adenomatous Polyposis Coli (APC) is a tumor suppressor gene. The APC gene encodes a large multifunctional protein that has critical functions in cell migration, adhesion and proliferation. It is a shuttling nucleo-cytoplasmic protein expressed in various tissues and its expression in the colorectal epithelium contributes to its normal growth and differentiation.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal region of human APC, conjugated to KLH. The corresponding sequence is identical in human APC isoform b and highly conserved (single amino acid substitution) in rat and mouse APC.

應用

Anti-APC (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunofluorescence
• immunohistochemistry

生化／生理作用

Adenomatous Polyposis Coli (APC) has a key role in the proteasome-mediated degradation of β-catenin. APC mutations cause accumulation of β-catenin in the nucleus leading to activation of LEF-1 and/or TCF, and the induction of target genes such as the oncogene c-myc. It is involved in regulation of mitotic chromosome separation and stability.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable