推薦產品
生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~250 kDa
物種活性
human, mouse
技術
indirect immunofluorescence: 10-20 μg/mL using human HeLa cells
western blot: 2.5-5 μg/mL using whole extracts of differentiated mouse C2 cells
western blot: 5-10 μg/mL using whole extracts of human HeLa cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... SVIL(6840)
mouse ... Svil(225115)
一般說明
Supervillin is an F-actin-binding protein originally isolated from bovine neutrophils. It has two isoforms of reported 205/250 kDa. The longer isoform named archvillin is muscle specific. Supervillin is a tightly bound peripheral membrane protein that is concentrated at sites of epithelial cell-cell adhesion. The COOH-terminus of supervillin is homologous to villin/gelsolin. The NH2-terminus contains functional nuclear localization sequences and F-actin and myosin II binding domains. Supervillin is found in many cells of several species, but is most abundant in muscle, bone marrow, thyroid gland and salivary gland.
免疫原
synthetic peptide corresponding to amino acid residues 900-918 of human supervillin with C-terminal added cysteine, conjugated to KLH. The corresponding sequence differs by two amino acids in mouse.
應用
Anti-Supervillin antibody produced in rabbit has been used in:
- immunoblotting
- chromatin immunoprecipitation (ChIP)
- immunofluorescence
生化／生理作用
Supervillin participates in cell-cell adhesion, motility regulation and information transfer between cell compartments. It has been suggested that supervillin may mediate actin and myosin II filament organization at cholesterol-rich membrane domains. Supervillin has been identified as a transcriptional activator of the androgen receptor. Increased levels of supervillin are found in many carcinoma cell lines, including HeLa S3 cervical carcinoma, SW480 adenocarcinoma and A549 lung carcinoma cells.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Archvillin, a muscle-specific isoform of supervillin, is an early expressed component of the costameric membrane skeleton
Oh SW, et al.
Journal of Cell Science, 116(11), 2261-2275 (2003)
F-actin and myosin II binding domains in supervillin
Chen Y, et al.
Test, 278(46), 46094-46106 (2003)
Supervillin associates with androgen receptor and modulates its transcriptional activity
Ting HJ, et al.
Proceedings of the National Academy of Sciences of the USA, 99(2), 661-666 (2002)
Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals
Wulfkuhle JD, et al.
Journal of Cell Science, 112(13), 2125-2136 (1999)
Proteomic analysis of a detergent-resistant membrane skeleton from neutrophil plasma membranes
Nebl T, et al.
The Journal of Biological Chemistry, 277(45), 43399-43409 (2002)
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