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重要文件

S2563

Sigma-Aldrich

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp.

同義詞:

SCDase

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About This Item

MDL號碼:
分類程式碼代碼:
12352211
NACRES:
NA.32
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異物活動

exoglycosidases: α- and β-galactosidase, α- and β-N-acetylgalactosaminidase, β-N-acetylglucosaminidase, α-mannosidase, α-fucosidase, and sialidase., essentially free
protease and sphingomyelinase, essentially free

品質等級

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. is a hydrolytic enzyme.[1]

應用

Sphingolipid Ceramide N-Deacylase from Pseudomonas sp. has been used in enzymatic conversion and derivatization of sulfatides.[2] It has also been used to hydrolyze the fatty acid chain of mactosyl ceramide.[3]

生化/生理作用

Hydrolyzes the N-acyl linkage between fatty acids and sphingosines in ceramides of various sphingolipids.
Sphingolipid Ceramide N-Deacylase from Pseudomonas sp., under certain conditions, can recyclate lyso-sphingolipids.[1] It condenses fatty acids to sphingosine to generate ceramide.[4] The enzyme mainly acts on neutral and acidic glycosphingolipids.[5]

單位定義

One unit will hydrolyze 1 μmol of asialo GM1 per minute at pH 6.0 at 37 °C.

外觀

Solution in 50 mM sodium acetate, pH 6.0, with 0.1% Lubrol PX.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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L Kuchar et al.
Rapid communications in mass spectrometry : RCM, 24(16), 2393-2399 (2010-07-17)
Sphingolipid ceramide N-deacylase (SCDase, EC 3.5.1.69) is a hydrolytic enzyme isolated from Pseudomonas sp. TK 4. In addition to its primary deacylation function, this enzyme is able to reacylate lyso-sphingolipids under specific conditions. We immobilised this enzyme on magnetic macroporous
Sulfatide Analysis by Mass Spectrometry for Screening of Metachromatic Leukodystrophy in Dried Blood and Urine Samples.
Spacil Z
Clinical Chemistry, 62(1), 279-286 (2016)
Characterization of the reversible nature of the reaction catalyzed by sphingolipid ceramide N-deacylase. A novel form of reverse hydrolysis reaction.
Kita K
European Journal of Biochemistry, 268(3), 592-602 (2001)
D Thusitha U Abeytunga et al.
Bioorganic chemistry, 36(2), 70-76 (2007-11-21)
Glycosphingolipids (GSLs) have been implicated as playing major roles in cellular interactions and control of cell proliferation in muticellular organisms. Moreover GSLs and other sphingolipids such as sphingomyelins, ceramides and sphingosines serve a variety of roles in signal transduction. Hence
A novel enzyme that cleaves the N-acyl linkage of ceramides in various glycosphingolipids as well as sphingomyelin to produce their lyso forms.
Ito M
The Journal of Biological Chemistry, 270(41), 24370-24374 (1995)

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