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RPROB

Sigma-Aldrich

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.32

用途

sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)

技術

protein staining: suitable

儲存溫度

2-8°C

相關類別

應用

R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.

其他說明

Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.

僅套裝組件

產品號碼
描述
  • Solution A 500 mL
  • Solution B 500 mL

象形圖

Corrosion
GHS05

訊號詞

Warning

危險聲明

H290,H315,H319

危險分類

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

儲存類別代碼

8B - Non-combustible corrosive hazardous materials

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)

文章

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

蛋白質染色試劑選擇指南

Sigma 提供 EZBlue™ 和 ProteoSilver™ 用於蛋白質可視化的試劑，適用於蛋白質組學和傳統 PAGE 格式。

條款

Introduction to Western Blot Detection of Proteins

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

蛋白質 Western Blot 檢測簡介

Western 印迹法包括通过凝胶电泳分离蛋白质，将蛋白质转移到聚偏二氟乙烯 (PVDF) 或硝酸纤维素膜上，并通过各种方法进行检测。

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