R3025

Anti-Rho-GDI antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution, ~1 mg/mL

• 同義詞: Anti-GDIA1, Anti-HEL-S-47e, Anti-NPHS8, Anti-RHOGDI, Anti-RHOGDI-1
• MDL號碼: MFCD01634148
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: IgG fraction of antiserum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~28 kDa
• 物種活性: mouse, bovine, human
• 濃度: ~1 mg/mL
• 技術: immunoprecipitation (IP): 4 μg using 500 μg of Jurkat RIPA lysate; western blot: 0.5-1.0 μg/mL using RIPA lysates from human HL-60, HeLa, Hs294T, Jurkat and murine 3T3/NIH cells and mouse 3T3/A31
• UniProt登錄號: P52565
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ARHGDIA(396) ; mouse ... Arhgdia(192662)

描述

一般說明

Rho guanine nucleotide dissociation inhibitor I (RhoGDI), also known as Rho GDP dissociation inhibitor α (ARHGDIA) is encoded by the gene mapped to human chromosome 17q25.3-25-3. RhoGDI is the member of Rho/Rac protein family. The encoded protein consists of 204 amino acids and has a molecular mass of 23,421,000 Mr. In mammals, three RhoGDI isoforms exists: RhoGDIα, RhoGDIβ and RhoGDIγ. RhoGDIα is widely expressed. RhoGDIβ is mainly expressed in hematopoietic cells and RhoGDIγ is present in the brain, lung, kidney and testis.

Rho proteins are basically described as guanine nucleotide-binding proteins (GNBPs). RhoGDI (Rho guanine nucleotide dissociation inhibitor)/ARHGDIA (Rho guanine nucleotide dissociation inhibitor) has three subtypes namely- RhoGDIα (Rho GDP dissociation inhibitor α), RhoGDIβ (Rho GDP dissociation inhibitor β) and RhoGDIγ (Rho GDP dissociation inhibitor γ). RhoGDIα is expressed everywhere. It is located on human chromosome 17q25.3. Its protein has 204 amino acids and belongs to the Rho/Rac family.

特異性

The antibody recognizes Rho-GDI and cross-reacts with D4-GDI.

免疫原

GST fusion protein corresponding to full-length human Rho-GDI.

應用

Anti-Rho-GDI antibody produced in rabbit has been used in immunoblotting.

Anti-Rho-GDI has been used in western blotting.

生化／生理作用

Rho proteins control the actin and microtubule cytoskeleton. RhoGDIα is necessary for the spatial and temporal modulation of RhoGNBPs (guanine nucleotide-binding proteins). In HCC (hepatocellular carcinoma) patients, absence of ARHGDIA (Rho guanine nucleotide dissociation inhibitor) affects the prognosis and induces invasion and metastasis of HCC cells.

外觀

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

準備報告

Purified using protein A

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable