PLA0071
Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified
Powered by Bethyl Laboratories, Inc.
同義詞:
32kDa, REPA2, RF-A protein 2, RP-A p32, RP-A p34, RPA32, replication factor A protein 2, replication protein A 34 kDa subunit, replication protein A2, replication protein A2 (32kD)
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About This Item
分類程式碼代碼:
12352203
NACRES:
NA.41
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生物源
rabbit
品質等級
抗體表格
affinity purified immunoglobulin
抗體產品種類
primary antibodies
物種活性
human, mouse
技術
immunocytochemistry: 1:500-1:5,000
immunohistochemistry: 1:500-1:2,000
immunoprecipitation (IP): 2-10 μg/mg
western blot: 1:2,000-1:10,000
登錄號
NP_002937.1
運輸包裝
wet ice
儲存溫度
2-8°C
目標翻譯後修改
phosphorylation (pSer4/pSer8)
基因資訊
human ... RPA2(6118)
一般說明
Replication protein A2 (RPA32) is encoded by the gene mapped to human chromosome 1p35.3. It is a subunit of the heterotrimeric replication protein A (RPA) complex.
免疫原
Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118).
生化／生理作用
Replication Protein A (RPA) binds single-stranded DNA and plays a vital role in DNA replication, DNA repair, and checkpoint signaling. Phosphorylation of replication protein A2 (RPA32) by ataxia telangiectasia and Rad3-related protein kinase (ATR) and DNA-dependent protein kinase (DNA-PK) causes checkpoint kinase 1 (Chk1) activation and replication arrest. DNA-PK and ATR signaling via RPA32 (S4/S8) enhances genome stability and cell survival following replication stress.
外觀
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
其他說明
Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
DNA-PK phosphorylation of RPA32 Ser4/Ser8 regulates replication stress checkpoint activation, fork restart, homologous recombination and mitotic catastrophe
Ashley AK, et al.
DNA Repair, 21(3), 131-139 (2014)
Bacterial DNA repair genes and their eukaryotic homologues: 4. The role of nucleotide excision DNA repair (NER) system in mammalian cells.
Maddukuri L, et al.
Acta Biochimica Polonica, 54(3), 469-482 (2007)
Radka Storchova et al.
The FEBS journal, 288(20), 6035-6051 (2021-05-14)
Upon exposure to genotoxic stress, cells activate DNA damage response (DDR) that coordinates DNA repair with a temporal arrest in the cell cycle progression. DDR is triggered by activation of ataxia telangiectasia mutated/ataxia telangiectasia and Rad3-related protein kinases that phosphorylate
Alina Vaitsiankova et al.
Nature structural & molecular biology, 29(4), 329-338 (2022-03-26)
Poly(ADP-ribose) polymerase 1 (PARP1) is implicated in the detection and processing of unligated Okazaki fragments and other DNA replication intermediates, highlighting such structures as potential sources of genome breakage induced by PARP inhibition. Here, we show that PARP1 activity is
George Rasti et al.
Nucleic acids research, 51(13), 6754-6769 (2023-06-13)
The Sirtuin family of NAD+-dependent enzymes plays an important role in maintaining genome stability upon stress. Several mammalian Sirtuins have been linked directly or indirectly to the regulation of DNA damage during replication through Homologous recombination (HR). The role of
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