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重要文件

P8498

Sigma-Aldrich

Monoclonal Anti-Profilin antibody produced in mouse

~2 mg/mL, clone 2-11H7 (MAbPRF12a), purified immunoglobulin, buffered aqueous solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203

生物源

mouse

共軛

unconjugated

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

2-11H7 (MAbPRF12a), monoclonal

形狀

buffered aqueous solution

分子量

antigen ~14 kDa

物種活性

Arabidopsis thaliana

濃度

~2 mg/mL

技術

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using extract of Arabidopsis sprout

同型

IgG1

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

Arabidopsis thaliana ... PFN2(829056) , PRF1(816495) , PRF3(835761)

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一般說明

Profilins are highly conserved pan-allergens that occur in plant foods, pollen and latex. Profilins have five distinct isoforms (PRF1-5) that bind with actin filaments to regulate cytoskeletal organization. PRF1, 2 and 3 are constitutive profilins that occur in all vegetative cells. During the early stages of microsporogenesis, these isoforms are also expressed in microspores and ovules . Monoclonal Anti-Profilin antibody is specific for Arabidopsis constitutive profilin (PRF1 and PRF2 and weakly with PRF3). The antibody also reacts with tobacco profilin (approx. 14 kDa).

免疫原

PRF1 protein.

應用

Monoclonal Anti-Profilin antibody is suitable for use in immunohistochemistry, western blot (1-2 μg/mL using extract of Arabidopsis sprout) and indirect ELISA.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Kandasamy, M.K., et al.
Cell Motility, 52, 115-126 (2002)
Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues
Kandasamy, M., K., et al.
Cell Motility, 52, 22-32 (2002)
Alexandra Santos et al.
International archives of allergy and immunology, 155(3), 191-204 (2011-02-05)
Profilins are ubiquitous proteins, present in all eukaryotic cells and identified as allergens in pollen, latex and plant foods. The highly conserved structure justifies the cross-reactive nature of IgE antibodies against plant profilins and their designation as pan-allergens. Primary sensitization

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