immunocytochemistry: suitable using 4% paraformaldehyde-0.5% Triton X-100 fixation indirect ELISA: suitable microarray: suitable western blot: 5-10 μg/mL using whole extract of rat brain
The PTEN (phosphatase and tensin homolog) gene is mapped to human chromosome 10q23. It shares sequence homology with protein tyrosine phosphatases and the cytoskeletal protein tensin.
特異性
The epitope recognized by the antibody resides within amino acids 386-403 of rat PTEN.
免疫原
synthetic peptide corresponding to the C-terminus of rat PTEN (amino acids 386-403).
生化／生理作用
The PTEN (phosphatase and tensin homolog) gene encodes a encodes a dual-specificity phosphatase that functions as a tumor suppressor by inhibiting phosphoinositide-3-kinase–protein kinase B/Akt (PI3K/PKB/Akt) signaling pathway, a crucial regulator of cell growth and survival and by negatively regulating the interactions of cell with the extracellular matrix. Mutations in this gene have been associated with several human cancers. Germline mutations in this gene have also been linked to Cowden disease (CD), Lhermitte-Duclos disease (LDD) and Bannayan-Zonana syndrome (BZS), which are characterized by increased susceptibility to tumor formation and developmental defects.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
The expression pattern of tumor suppressor gene phosphatase and tensin homolog deleted on chromosome ten (PTEN) and phosphatase and tensin homolog deleted on chromosome ten/phosphatidylinositol3-kinase/protein kinase B (PTEN/PI3K/AKT) cell signaling pathway in renal cell carcinoma (RCC) were investigated in children.
