This product can be used to remove endotoxins from solutions. A protocol for use and information on regeneration of the resin are provided in the Product Information Sheet.
biological source
synthetic (organic)
Quality Segment
form
aqueous glycerol suspension
extent of labeling
~1 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
200-500 μg/mL binding capacity (lipopolysaccharide)(from Escherichia coli serotype 0128:B12)
suitability
suitable for chromatography
storage temp.
2-8°C
Application
可用于从溶液中去除内毒素
多粘菌素 B-琼脂糖用于蛋白质色谱、亲和色谱和专业树脂。多粘菌素 B-琼脂糖已用于研究牙周炎和牙髓感染，以及肥胖和 2 型糖尿病。
Physical form
悬浮于含有 0.02％ 叠氮化钠的 50％ 甘油中
1 of 1
本產品 | |||
|---|---|---|---|
| description aqueous glycerol suspension | description cationic cyclic peptide | description standard for GC | description For fluorescent microbial imaging, 1.1 mg/mL in water |
| biological source synthetic (organic) | biological source - | biological source - | biological source - |
| technique(s) affinity chromatography: suitable | technique(s) - | technique(s) gas chromatography (GC): suitable, HPLC: suitable | technique(s) - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form aqueous glycerol suspension | form lyophilized powder | form - | form - |
| suitability suitable for chromatography | suitability - | suitability - | suitability - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. −20°C |
存儲類別/等級
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
-
What is the application of this polymyxin-B agarose? can we re-use it for several times? And if yes, how can we regenerate it? thanks!
1 answer-
Helpful?
-
-
What polypropylene column should the we use according to the protocol to wash the resin prior to use?
1 answer-
For washing the resin prior to use, the customer can use the following options:
1. C2603 Mini-Columns: These have a capacity of 0.5 mL and an overall height of 1.9 inches.
2. C2728 Mini-Columns: These have a capacity of 2.4 mL and an overall height of 1.5 inches.
The washing procedure using Polymyxin B Agarose (Product No. P1411) can be carried out in a column or in a batch method. For a column, the customer can use disposable mini columns, such as Product Nos. C2603 (0.5 ml) and C2728 (5.0 ml). If a larger column is needed, the customer can use a polypropylene syringe barrel or use the batch method.
In the batch method, the solution is incubated with the polymyxin B agarose for a minimum of 30 minutes with rotation in either a 15 ml or 50 ml polypropylene tube. The tube is then centrifuged, and the supernatant contains the protein of interest with decreased endotoxin that has been bound to the beads and pelleted in the tube.
Helpful?
-
-
What methods can be used to remove endotoxin from a product?
1 answer-
The product is not tested for endotoxin; however, P1411 may be used to strip endotoxin. The P1411 datasheet includes a suggested protocol for this process. The protocol involves washing the resin, equilibrating the column, dissolving the sample in buffer, eluting with endotoxin-free buffer, and collecting the fractions. The collected fractions containing the protein should then be lyophilized. The polymyxin B agarose can be regenerated at least 10 times, and the resin can be stored in 25% ethanol at 4°C
Helpful?
-