Poly-D-lysine hydrobromide with a molecular weight greater than 30,000 Da is recommended for cell attachment. The poly-D-lysine hydrobromide with a molecular weight of 84,000 Da has more attachment sites per polymer chain available to the cells. This type is most suitable for cell attachment.
推薦產品
產品名稱
聚-D-赖氨酸 氢溴酸盐, mol wt ≥300,000
形狀
powder or solid
品質等級
分子量
≥300,000
技術
cell culture | mammalian: suitable
顏色
white to off-white
儲存溫度
−20°C
SMILES 字串
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI 密鑰
MEXAGTSTSPYCEP-UHFFFAOYSA-N
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應用
聚-D-赖氨酸聚合物可用于制备细胞附着的表面。D-赖氨酸聚合物也可用于消化聚-L-赖氨酸聚合物并导致L-赖氨酸过量摄取的细胞。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
建议使用0.5 - 1.0 mL的该产品0.1 mg/mL溶液用作细胞培养的基质对25 cm2进行涂覆。 较低分子量的该产品粘度相对较低，但较高分子量的形式则可提供每分子更多的附着位点。
生化／生理作用
多聚-L-赖氨酸（PDL）氢溴酸盐是细胞的非特异性粘附因子，它通过增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用，促进细胞与固相基质的粘附。 当被细胞培养表面吸收后，聚-D-赖氨酸可增加带正电的细胞结合位点数量。
成分
聚-D-赖氨酸是一种带正电的氨基酸聚合物，其中每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-D-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。
注意
无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。
準備報告
本品分子量 > 30 万。为除去 HBr，将本品溶于中性缓冲液中，透析除去盐类。一般以本品为附着因子，在 5 mg 聚赖氨酸中加入 50 mL 无菌组织培养级水，每 25 cm2 溶液无菌涂布表面 1 mL。5 min 后，通过抽吸清除溶液，并彻底冲洗表面。在引入细胞和培养基前干燥两小时。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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分析證明 (COA)
Lot/Batch Number
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Humankind has utilized protein materials throughout its existence, starting with the use of materials such as wool and silk for warmth and protection from the elements and continuing with the use of recombinant DNA techniques to synthesize proteins with unique and useful properties.
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What type of Poly-D-lysine hydrobromide is most suitable for cell attachment?
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