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OGS590

Sigma-Aldrich

PUC19 - 高拷贝蓝/白克隆质粒

plasmid vector for molecular cloning

同義詞:

snapfast 载体

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.85
暫時無法取得訂價和供貨情況

形狀

buffered aqueous solution

分子量

size 2686 bp

菌種選擇

ampicillin

複製起點

pUC (500 copies)

肽切割

no cleavage

啟動子

Promoter name: Lac
Promoter activity: inducible
Promoter type: bacterial

報告基因

none

運輸包裝

ambient

儲存溫度

−20°C

一般說明

这种高拷贝数质粒是通用的工业标准克隆载体,可用于使用蓝/白筛选进行插入和亚克隆基因。

應用

基因克隆:PUC19 - 高拷贝蓝/白克隆质粒是数千个实验室在常规克隆中使用的标准克隆质粒。该质粒可用于蓝白筛选,并含有多个多克隆位点,其从EcoRI延伸至Hind3限制性位点,允许插入DNA序列。

序列

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分析報告

欲查看本品的分析证书,请访问 www.oxgene.com

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描述
訂價

儲存類別代碼

12 - Non Combustible Liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown.
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual

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