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Merck
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重要文件

MAK450

Sigma-Aldrich

Glucose Dehydrogenase Assay Kit

Sufficient for 100 colorimetric tests

同義詞:

GDH Activity Assay Kit

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84
暫時無法取得訂價和供貨情況

品質等級

儲存條件

(No metal containers. Keep container tightly closed in a dry and well -ventilated place. Keep away from heat and sources of ignition.)

輸入

tissue
cell culture medium
tissue
plasma

檢測方法

colorimetric

相關疾病

endocrinological disorders, diabetes; hematological disorder

儲存溫度

−20°C

一般說明

Glucose Dehydrogenase (GDH) belongs to the family of oxioreductases, specifically those acting on the CH-OH group of donor with other acceptors. GDH participates in the pentose phosphate pathway.

應用

The Glucose Dehydrogenase Assay Kit may be used for:
  • Metabolism Research
  • Drug Discovery

特點和優勢

  • Efficient and Precise: Requires only 20 μL of sample and a short 15-minute reaction time while detecting glucose dehydrogenase levels across a broad range from 0.5 to 200 U/L, providing both convenience and sensitivity.
  • Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

適合性

The kit is suitable for the quantitative determination of D-glucose dehydrogenase enzyme activity and drug effects on its metabolism in biological samples (e.g. plasma, serum, tissue and culture media).

原則

The Glucose Dehydrogenase Activity Assay Kit is a non-radioactive, colorimetric GDH assay. It is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A565 nm. The increase in absorbance at A565 nm is proportional to the enzyme activity.

其他說明

For additional information on our range of Biochemicals, please complete this form.

訊號詞

Danger

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

3 - Flammable liquids


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