M6320

Monoclonal Anti-MAGE-1 小鼠抗

~2 mg/mL, clone MA454, purified immunoglobulin, buffered aqueous solution

• 同義詞: Anti-Melanoma-associatiated Antigen 1
• MDL號碼: MFCD03096998
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: MA454, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~46 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 濃度: ~2 mg/mL
• 技術: immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 5-10 μg/mL using total cell extract of SKML37 cells
• 同型: IgG1
• UniProt登錄號: P43355
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MAGEA1(4100)

描述

一般說明

Melanoma-associatiated antigen 1 (MAGE-1) is part of the CG (cancer-germline) group of genes and is a tumor-specific antigen.[1][2] MAGE-1 is a member of the MAGE-A family. MAGE-1 gene is localized on human chromosome Xq28.[3] Monoclonal Anti-MAGE-1 (mouse IgG1 isotype) is derived from the hybridoma MA454 produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from mice immunized with human recombinant MAGE-1.

特異性

The antibody does not recognize MAGE-2 and MAGE-3.[4][5]

免疫原

recombinant human MAGE-1.

應用

Monoclonal Anti-MAGE-1 antibody produced in mouse has been used in:

• ELISA
• immunoblotting
• immunohistochemistry

生化／生理作用

The melanoma antigen genes (MAGE) might play a key role in neural development and apoptosis. Expression of melanoma-associated antigen 1 (MAGE-1) in neoplastic cells depends on the binding of the transcription factor Ets to non-methylated cis regulatory sequences, located in its promoter. MAGE-1 expression is mediatedby the B and B′ domains of MAGE-A1 gene. Expression of MAGE-1A was induced in non MAGE-A1 expressing tumor cells once treated with demethylating reagent, 5-aza-2-(5DC).[6] Melanoma-associatiated antigen 1 (MAGE-1) has been shown to be expressed in different types of cancers.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)