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M2378

Sigma-Aldrich

黏液蛋白 来源于猪胃

Type II

同義詞:

MUC

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About This Item

CAS號碼:
84082-64-4
EC號碼:
282-010-7
MDL號碼:
MFCD00131629
分類程式碼代碼:
12352202
NACRES:
NA.61
暫時無法取得訂價和供貨情況

生物源

Porcine stomach

種類

Type II

形狀

powder

成份

bound sialic acid, ≤1.2%

技術

cell culture | mammalian: suitable

溶解度

1 M NaOH: soluble 20 mg/mL

UniProt登錄號

A0A5G2QSD1

儲存溫度

2-8°C

基因資訊

pig ... MUC2(100127145)

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一般說明

粘蛋白主要由碳水化合物单元组成，对结构结构和构象至关重要。[1]粘蛋白的单体为640 kDa，蛋白质仅占分子量的20％。其中的碳水化合物成分附着在苏氨酸和丝氨酸侧链上。[2]粘蛋白分为分泌型和跨膜粘蛋白。[3]
黏液蛋白或黏液糖蛋白是哺乳动物黏液的主要大分子成分。[4]它还被应用于评价细胞离心浓缩痰涂片检测抗酸杆菌的研究。[5]

應用

来自猪胃的粘蛋白以用于：
  • 绘制定量小鼠粘膜炎症过程产生的粘蛋白2的标准曲线[6]
  • 用作人体外消化模型中的消化液溶液组分[7]
  • 用作模拟唾液的组分[8]

猪胃黏液素已应用于 铜绿假单胞菌半乳糖特异性凝集素PA-IL结合位点的研究。[9]

生化／生理作用

粘蛋白在药物递送应用中具有广泛的药理学应用。[1]粘蛋白在炎症过程中起着保护作用。粘蛋白合成失调有可能引起肿瘤发生和慢性炎症。[3]
黏液形成所有上皮表面的保护层。黏液的主要结构成分是形成凝胶的黏液素。覆盖肠上皮的黏液层以MUC2粘蛋白为中心分子。MUC2是由杯状细胞产生。[10]

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
0000434623
0000436335
0000436335
0000434623
0000426104

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存取文件庫

Mucins in cancer: function, prognosis and therapy
Kufe DW
Nature Reviews. Cancer, 9(12), 874-874 (2009)
An optimized purification process for porcine gastric mucin with preservation of its native functional properties
Schomig VJ, et al.
Royal Society of Chemistry Advances, 6(50), 44932-44943 (2016)
Structural investigation of porcine stomach mucin by X-ray fiber diffraction and homology modeling
Veluraja K, et al.
Biochemical and biophysical research communications, 406(4), 570-573 (2011)
Enhancement of the solubility, stability and bioaccessibility of quercetin using protein-based excipient emulsions
Chen X, et al.
Food Research International, 114, 30-37 (2018)
Amparo Gamero et al.
Foods (Basel, Switzerland), 8(5) (2019-05-06)
The aim of the present study was to determine how variation in the biscuit matrix affects both the degree of in vitro fragmentation and the starch hydrolysis that occurs during the oral phase of digestion. Using three different oat ingredient
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文章

Sialic Acid

Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.

醯胺酸

瞭解半乳醯胺酸的結構、功能、訊號傳遞及變性。輕鬆尋找研究ialic acid 的產品。

Questions

1–9 of 9 Questions  

  1. What is the best way to sterilise the product without destroying the structure?

    1 answer

    1. The structural integrity of this product has not been evaluated post-sterilization. A general procedure for sterilizing mucin has been published as follows:
      1. Place dry powdered mucin in a flask.
      2. Cover the mucin with 95% ethanol.
      3. Heat the covered mucin at 70 °C for 24 hours.
      4. Evaporate the alcohol to recover the mucin.
      This information is taken from Yeung, A.T.Y. et al., MBio, 3(3), e00073-12 (2012).

      In addition, this product is described as soluble in 1 M NaOH: soluble 20 mg/mL:
      https://www.sigmaaldrich.com/product/sigma/m2378

      This Millex syringe filter should be chemically compatible to filter sterilize that solution (this product is intended for a volume range ~ 10 – 100 ml solution):
      https://www.sigmaaldrich.com/product/mm/slgvr33rs

      See user guide:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/409/570/pr05510w-ms.pdf

      Helpful?

  2. How does this differ from human mucin?

    1 answer

    1. This product has not been tested in comparison to human-source mucin.
      It may be helpful to read through the following publication:
      https://www.nature.com/articles/s41598-020-77129-4

      Helpful?

  3. I saw that this product is made by peptide degradation. by that way, can it harvest transmembrane mucins? I wonder this product contains transmembrane mucin too, especially MUC1.

    1 answer

    1. This product is a very crude preparation of mucin obtained by digestion of hog stomach with pepsin, followed by precipitation of the mucin with alcohol. The crude mucin is collected by filtration and dried. It is a mixture of glycoproteins. Transmembrane mucins tend to be heavily glycosylated and are more resistant to digestion however, conditions such as pH, temperature, and activity of the enzyme may lead to degradation. Further characterization of the mucins present in the final product is not performed.

      Helpful?

  4. What is the molecular weight of Product M2378, Mucin from porcine stomach?

    1 answer

    1. This product is a crude mixture of glycoproteins. The sugar and amino acid composition of the mucin has not been determined.

      Helpful?

  5. How can I solubilize Product M2378, Mucin from porcine stomach?

    1 answer

    1. This crude mucin may not be readily soluble in solution. At 20 mg/mL in 1 M sodium hydroxide, turbid suspensions are observed. To try to increase the solubility, one might try further dilution and sonication, but we have not tried this in our labs.

      Helpful?

  6. What are the differences between Mucin from porcine stomach Products M2378 and M1778?

    1 answer

    1. Product No. M2378 is a crude mucin preparation. Product No. M1778 is a partially purified powder prepared according to the method described in Glenister, D.A. and Salmon. K. Microbial Ecol. in Health & Disease 1, 31, (1988). The amount of bound sialic acids are also different. Please refer to the specification sheets on our website.

      Helpful?

  7. What does the "Type" designation in Mucin from porcine stomach mean?

    1 answer

    1. The type designation does not have any particular biological significance.  It is used to emphasize differences in the listings of this product.

      Helpful?

  8. Is Product M2378, Mucin from porcine stomach, a purified preparation of mucin?

    1 answer

    1. No. This product is a crude preparation of mucin; it is a mixture of glycoproteins.  It is obtained by digestion of hog stomach with pepsin, followed by precipitation and other steps.

      Helpful?

  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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