L4418

Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-Amine oxidase, flavin-containing, 2, Anti-BRAF-HDAC complex protein 110, Anti-KIAA0601 protein, Anti-Lysine-specific demethylase 1, Anti-NPAO, Anti-Nuclear Polyamine Oxidase
• MDL號碼: MFCD09039194
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 110-115 kDa
• 物種活性: human, mouse (predicted), rat (predicted)
• 濃度: ~1 mg/mL
• 技術: immunocytochemistry: 5-10 μg/mL using MCF7 cells fixed with formaldehyde; immunoprecipitation (IP): 1-2 μg using MCF7 cell lysates; western blot: 1-2 μg/mL using MCF7 cell lysates (2 bands, 2 isoforms)
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... AOF2(23028) ; mouse ... Aof2(99982)

描述

一般說明

Lysine specific demethylase 1 (LSD1) also known as amine oxidase flavin containing domain 2 (AOF2) is a lysine specific demethylase. It is a nuclear protein containing a Swi3p, Rsc8p and Moira (SWIRM) domain, a flavin adenine dinucleotide (FAD)-binding motif and an amine oxidase domain.

免疫原

synthetic peptide corresponding to amino acids 835-852 of human LSD1 (AOF2) (isoform b), conjugated to KLH via an N-terminal added cysteine reisidue. The immunizing sequence is present also in isoform a. This sequence is conserved in human, rat, and mouse.

應用

Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit immunoblotting, immunoprecipitation and immunofluorescence.

生化／生理作用

Lysine specific demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription. LSD1 (AOF2) action can signal either activation or repression of transcription. Demethylation of histone H3 Lys4 by LSD1 (AOF2) leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to de-repression of androgen receptor target genes. The activity of LSD1 (AOF2) is regulated by its associated factors. REST corepressor 1 (CoREST), a transcriptional corepressor, promotes demethylation by enhancing the association of LSD1 (AOF2) with the nucleosomes, and it also protects LSD1 (AOF2) from proteasomal degradation.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable