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Merck
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重要文件

L1668

Sigma-Aldrich

来自 菜豆的凝集素 （红芸豆）

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

同義詞:

PHA, 植物血球凝集素(菜豆)

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About This Item

分類程式碼代碼:
12352202
NACRES:
NA.32
暫時無法取得訂價和供貨情況

品質等級

200

產品線

BioReagent

形狀

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

效力

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

技術

cell culture | mammalian: suitable

顏色

white to off-white

溶解度

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

儲存溫度

2-8°C

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相關類別

應用

来自菜豆（Phaseolus vulgaris） （红芸豆）的凝集素已被用于：
  • 刺激分化簇（CD4+）T细胞[1]
  • 激活原发性外周血淋巴细胞[2]
  • 刺激外周血单核细胞[3][4]

生化／生理作用

PHA由两种分子种类组成，即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素（PHA-E），和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素（PHA-L）。
PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

包裝

根据凝集素的含量进行出售

分析報告

其效力和溶解度以mg凝集素/ml表示

相關產品

產品號碼
描述
訂價

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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分析證明 (COA)

Lot/Batch Number
0000408863
0000406766
0000384022
0000384022
0000408863

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Sarracino A, et al.
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文章

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Questions

1–7 of 7 Questions  

  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer

    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

      Helpful?

  2. How should the PHA solution using Phytohemagglutinin PHA-P powder be prepared for cell culture?

    1 answer

    1. The stock solution of this product can be prepared in phosphate buffered saline at pH 6.8, resulting in a clear to slightly hazy solution at a concentration of 5 mg per mL. This stock solution can then be diluted in cell culture medium to a working concentration of 10 micrograms of lectin per mL for use in peripheral blood cell culture.

      Helpful?

  3. What is the shelf life once reconstituted in PBS?

    1 answer

    1. After reconstitution in PBS, this product can be stored at 2-8°C for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions of at least 1 mg/mL can be stored in frozen aliquots. Do not dilute to the working concentration until ready to use. Vortex stock solutions prior to use.

      Helpful?

  4. What is the solution stability for Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer

    1. The solution stability was not evaluated by Sigma-Aldrich; however, references indicate media containing dilute PHA can be stored refrigerated for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions can be stored in frozen aliquots.

      Helpful?

  5. How can Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder, be dissolved?

    1 answer

    1. Product No. L1668 is solubility tested using 5 mg per mL of phosphate buffered saline (PBS), pH 6.8; this yields a clear to slightly hazy, colorless to light yellow solution.

      Helpful?

  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is the mitogenic activity of Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer

    1. The minimum specification for mitogenic activity of this product is  < or = 10 micrograms lectin/mL of medium. The reported specification is PASS. A specific value is not given.

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