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Merck
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I2771

Sigma-Aldrich

ITS+3 液体培养基补充剂 (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

同義詞:

ITS Supplement

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About This Item

分類程式碼代碼:
12352205
NACRES:
NA.75

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

運輸包裝

ambient

儲存溫度

2-8°C

相關類別

一般說明

ITS+3液体培养基补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠、亚油酸和牛血清白蛋白(BSA)的混合物。适合无血清细胞培养应用。

應用

ITS+3液体培养基补充剂(100×)作为培养基补充剂已用于：
  • 支持人脂肪干细胞(ASC)生长
  • 培养猪椎间盘(IVD)细胞和组织
  • 培养软骨终板(CEP)细胞

生化／生理作用

  • 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，可能会影响有丝分裂。
  • 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了滋养培养细胞，可与血清转铁蛋白结合后使用。
  • 硒：血清中的一种必需微量元素。

成分

含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、470 μg/m亚油酸、470 μg/ml 油酸和50 mg/ml牛血清白蛋白（100x 浓度）。每摩尔白蛋白含有 2 摩尔亚油酸和油酸。

準備報告

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。

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Adam Cornish et al.
BMC genomics, 21(Suppl 11), 830-830 (2020-12-30)
Single-cell sequencing enables us to better understand genetic diseases, such as cancer or autoimmune disorders, which are often affected by changes in rare cells. Currently, no existing software is aimed at identifying single nucleotide variations or micro (1-50 bp) insertions and
Xue Liu et al.
iScience, 24(6), 102551-102551 (2021-06-22)
Pulmonary mesenchymal cells are critical players in both the mouse and human during lung development and disease states. They are increasingly recognized as highly heterogeneous, but there is no consensus on subpopulations or discriminative markers for each subtype. We completed
Katherine Lakstins et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(6), 1204-1216 (2020-04-15)
Cartilage endplate degeneration/calcification has been linked to the onset and progression of intervertebral disc degeneration and there is a critical need to understand mechanisms, such as hypertrophic differentiation, of cartilage endplate degeneration/calcification to inform treatment strategies for discogenic back pain.
Di Wu et al.
International journal of stem cells, 14(1), 119-126 (2020-12-31)
The research on human hepatobiliary development and disorders has been constrained by minimal access to human fetal tissue, and low accuracy of animal models. To overcome this problem, we have established a system for the differentiation of human pluripotent stem
Hien Lau et al.
Islets, 12(3), 41-58 (2020-05-28)
The use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM)
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