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H8280

Sigma-Aldrich

N-羟基琥珀酰亚胺基琼脂糖凝胶 4 快 慢

matrix fast-flow highly cross-linked 4% beaded agarose

同義詞:

N -羟基琥珀酰亚胺-琼脂糖

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About This Item

MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

形狀

isopropanol suspension

標籤範圍

16-23 μmol per mL

技術

protein array: suitable

基質

fast-flow highly cross-linked 4% beaded agarose

基質活性組

N-hydroxysuccinimide active ester

基質墊片

6 atoms (when ligands are coupled by displacement of NHS)

儲存溫度

2-8°C

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一般說明

N-羟基琥珀酰-琼脂糖 4 Fast Flow是一种交联预活化的基质,通过间隔臂将Sepharose 4 Fast Flow与6-氨基己酸混合获得。与N-羟基琥珀酰的酯化反应激活了末端羧基。含有配体的基直接与该活性酯相互作用,形成化学性质非常稳定的酰胺键。

應用

N-羟基琥珀酰-琼脂糖 4 Fast Flow已被用作基于磁颗粒的视觉检测系统的一部分,以实现扩增基因结合后的分离。它也被用来固定蛋白质在拉下试验。

特點和優勢

  • 特别推荐用于工艺规模应用中含氨基的小蛋白和多肽的偶联
  • 高水平的预激活,这提供了所选配体的高度替代
  • NHS偶联方法使配体的化学稳定附着

法律資訊

Sepharose is a trademark of Cytiva

象形圖

FlameExclamation mark

訊號詞

Danger

危險聲明

危險分類

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

3 - Flammable liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

53.6 °F

閃點(°C)

12 °C

個人防護裝備

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


分析證明 (COA)

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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(2), 361-363 (2004-05-12)
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Yoo Min Park et al.
Biosensors & bioelectronics, 141, 111415-111415 (2019-06-16)
We focused on the development of a hand-held pathogen-detection device using smartphone-embedded electronic elements combined with functionalized magnetic particles (MPs) and sepharose. To perform affinity chromatography for evaluating DNA amplicons, avidin-conjugated MPs and succinimide-linked sepharose were used with biotin-primers. To
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The current study focuses on developing a system for visually detecting an amplified bacterial (Escherichia coli O157:H7) gene using a heavy metal particle (MP) and functionalized porous sepharose gel. To functionalize DNA-specificity to the MP, an avidin-modified MP was employed
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