GE28-9782-43

HiScreen^™ Blue Fast Flow

Cytiva 28-9782-43

• 分類程式碼代碼: 13111000
• NACRES: NA.56

屬性

• ligand: Cibacron Blue
• 包裝: pkg of 1 ea
• 製造商／商標名: Cytiva 28-9782-43
• 儲存條件: (20% Ethanol, 0.1 M potassium phosphate); (20% Ethanol, 0.9% NaCl, 1 mM Ca2₊ and 1 mM Mn2₊)
• 參數: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• 床尺寸: 7.7 mm × 100 mm
• 床體積: 4.7 mL
• 柱內徑: 7.7 mm
• 基質: 6% cross-linked agarose
• 粒徑: 45-165 μm
• 平均直徑: 90 μm
• cleaning: 3-13
• 工作範圍: 4-12
• 適合性: suitable for bioprocess medium
• 儲存溫度: 2-8°C

描述

一般說明

HiScreen^™ columns prepacked with Blue Sepharose^™ Fast FLow are an excellent choice for method optimization and parameter screening for purification of albumin, enzymes (including NAD+ and NADP+), coagulation factors, interferons, and related proteins.

應用

HiScreen^™ columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen^™ columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The media used in HiScreen^™ columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen^™ Blue FF is prepacked with the BioProcess chromatography medium Blue Sepharose^™ 6 Fast Flow. This medium has Cibacron Blue 3G covalently attached to the Sepharose^™ 6 Fast FLow matrix by the triazine coupling method. The Blue dye binds many Proteins, such as albumin, interferon, lipoProteins, and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors (e.g., NAD).

特點和優勢

• Can be used for depletion of albumin.
• Prepacked, ready-to-use columns for convenient process development.
• Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
• Easy connection in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全資訊

• 象形圖: GHS02
• 訊號詞: Warning
• 危險聲明: H226
• 防範說明: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 儲存類別代碼: 3 - Flammable liquids