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GE28-9670-56

Protein A Mag Sepharose™ Xtra

Cytiva 28-9670-56, pack of 2 × 1 mL

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56
暫時無法取得訂價和供貨情況

ligand

native protein A

包裝

pack of 2 × 1 mL

製造商／商標名

Cytiva 28-9670-56

儲存條件

(20% Ethanol, 10% medium slurry)

參數

Room temperature temp. range

基質

paramagnetic, spherical, highly cross-linked agarose particles

容量

27 mg binding capacity(human IgG/ml gel)

儲存溫度

2-8°C

相關類別

一般說明

protein A Mag Sepharose™ are magnetic beads designed to simplify small-scale purification and screening of antibodies. Based on Sepharose™ with native protein A as ligand.

應用

Protein A Mag Sepharose™ Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG/mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Robust parallel screening of antibodies with high reproducibility.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device.

特點和優勢

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized high capacity for efficient small-scale purification and screening of antibodies from various species
  • High purity and yield

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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分析證明 (COA)

Lot/Batch Number

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Lukas Leiendecker et al.
EMBO molecular medicine, 12(11), e12525-e12525 (2020-10-08)
Merkel cell carcinoma (MCC) is a highly aggressive, neuroendocrine skin cancer that lacks actionable mutations, which could be utilized for targeted therapies. Epigenetic regulators governing cell identity may represent unexplored therapeutic entry points. Here, we targeted epigenetic regulators in a
Qingzhou Ji et al.
Nucleic acids research, 42(10), 6158-6167 (2014-05-06)
Artificial transcription factors are powerful tools for regulating gene expression. Here we report results with engineered zinc-finger transcription factors (ZF-TFs) targeting four protein-coding genes, OCT4, SOX2, KLF4 and c-MYC, and one noncoding ribonucleic acid (RNA) gene, the microRNA (miRNA) miR302/367

文章

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

免疫沉澱技術

本頁說明免疫沉淀（免疫親和或拉取技術）。

抗體親和層析的脫鹽和緩衝液交換

在實驗室規模下進行脫鹽是一種行之有效、簡單且快速的方法，可快速去除低分子量雜質，同時只需一個步驟即可將樣品轉移到所需的緩衝液中。

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Purification of IgG Antibodies with Mag Sepharose®

Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra enable high-capacity small-scale antibody purification and screening.

使用 Mag Sepharose® 純化 IgG 抗體

Protein A Mag Sepharose® Xtra 和 Protein G Mag Sepharose® Xtra 可實現高容量的小規模抗體純化和篩選。

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